Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly
© 2014 Shen et al..
The scission of biological membranes is facilitated by a variety of protein complexes that bind and manipulate lipid bilayers. ESCRT-III (endosomal sorting complex required for transport III) filaments mediate membrane scission during the ostensibly disparate processes of multivesicular endosome biogenesis, cytokinesis, and retroviral budding. However, mechanisms by which ESCRT-III subunits assemble into a polymer remain unknown. Using cryogenic electron microscopy (cryo-EM), we found that the full-length ESCRT-III subunit Vps32/CHMP4B spontaneously forms single-stranded spiral filaments. The resolution afforded by two-dimensional cryo-EM combined with molecular dynamics simulations revealed that individual Vps32/CHMP4B monomers within a filament are flexible and able to accommodate a range of bending angles. In contrast, the interface between monomers is stable and refractory to changes in conformation. We additionally found that the carboxyl terminus of Vps32/CHMP4B plays a key role in restricting the lateral association of filaments. Our findings highlight new mechanisms by which ESCRT-III filaments assemble to generate a unique polymer capable of membrane remodeling in multiple cellular contexts.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:206 |
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| Enthalten in: |
The Journal of cell biology - 206(2014), 6 vom: 15. Sept., Seite 763-77 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shen, Qing-Tao [VerfasserIn] |
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| Links: |
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| Themen: |
Endosomal Sorting Complexes Required for Transport |
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| Anmerkungen: |
Date Completed 18.11.2014 Date Revised 21.10.2021 published: Print-Electronic PDB: 2GD5 Citation Status MEDLINE |
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| doi: |
10.1083/jcb.201403108 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM241719240 |
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| 100 | 1 | |a Shen, Qing-Tao |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly |
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| 500 | |a Date Completed 18.11.2014 | ||
| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a PDB: 2GD5 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2014 Shen et al. | ||
| 520 | |a The scission of biological membranes is facilitated by a variety of protein complexes that bind and manipulate lipid bilayers. ESCRT-III (endosomal sorting complex required for transport III) filaments mediate membrane scission during the ostensibly disparate processes of multivesicular endosome biogenesis, cytokinesis, and retroviral budding. However, mechanisms by which ESCRT-III subunits assemble into a polymer remain unknown. Using cryogenic electron microscopy (cryo-EM), we found that the full-length ESCRT-III subunit Vps32/CHMP4B spontaneously forms single-stranded spiral filaments. The resolution afforded by two-dimensional cryo-EM combined with molecular dynamics simulations revealed that individual Vps32/CHMP4B monomers within a filament are flexible and able to accommodate a range of bending angles. In contrast, the interface between monomers is stable and refractory to changes in conformation. We additionally found that the carboxyl terminus of Vps32/CHMP4B plays a key role in restricting the lateral association of filaments. Our findings highlight new mechanisms by which ESCRT-III filaments assemble to generate a unique polymer capable of membrane remodeling in multiple cellular contexts | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 7 | |a Endosomal Sorting Complexes Required for Transport |2 NLM | |
| 650 | 7 | |a Polymers |2 NLM | |
| 650 | 7 | |a Protein Subunits |2 NLM | |
| 700 | 1 | |a Schuh, Amber L |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Yuqing |e verfasserin |4 aut | |
| 700 | 1 | |a Quinney, Kyle |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lei |e verfasserin |4 aut | |
| 700 | 1 | |a Hanna, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Mitchell, Julie C |e verfasserin |4 aut | |
| 700 | 1 | |a Otegui, Marisa S |e verfasserin |4 aut | |
| 700 | 1 | |a Ahlquist, Paul |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Audhya, Anjon |e verfasserin |4 aut | |
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