A Yersinia pestis tat mutant is attenuated in bubonic and small-aerosol pneumonic challenge models of infection but not as attenuated by intranasal challenge
Bacterial proteins destined for the Tat pathway are folded before crossing the inner membrane and are typically identified by an N-terminal signal peptide containing a twin arginine motif. Translocation by the Tat pathway is dependent on the products of genes which encode proteins possessing the binding site of the signal peptide and mediating the actual translocation event. In the fully virulent CO92 strain of Yersinia pestis, the tatA gene was deleted. The mutant was assayed for loss of virulence through various in vitro and in vivo assays. Deletion of the tatA gene resulted in several consequences for the mutant as compared to wild-type. Cell morphology of the mutant bacteria was altered and demonstrated a more elongated form. In addition, while cultures of the mutant strain were able to produce a biofilm, we observed a loss of adhesion of the mutant biofilm structure compared to the biofilm produced by the wild-type strain. Immuno-electron microscopy revealed a partial disruption of the F1 antigen on the surface of the mutant. The virulence of the ΔtatA mutant was assessed in various murine models of plague. The mutant was severely attenuated in the bubonic model with full virulence restored by complementation with the native gene. After small-particle aerosol challenge in a pneumonic model of infection, the mutant was also shown to be attenuated. In contrast, when mice were challenged intranasally with the mutant, very little difference in the LD50 was observed between wild-type and mutant strains. However, an increased time-to-death and delay in bacterial dissemination was observed in mice infected with the ΔtatA mutant as compared to the parent strain. Collectively, these findings demonstrate an essential role for the Tat pathway in the virulence of Y. pestis in bubonic and small-aerosol pneumonic infection but less important role for intranasal challenge.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2014 |
---|---|
Erschienen: |
2014 |
Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
---|---|
Enthalten in: |
PloS one - 9(2014), 8 vom: 06., Seite e104524 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Bozue, Joel [VerfasserIn] |
---|
Links: |
---|
Themen: |
Aerosols |
---|
Anmerkungen: |
Date Completed 30.11.2015 Date Revised 21.10.2021 published: Electronic-eCollection Citation Status MEDLINE |
---|
doi: |
10.1371/journal.pone.0104524 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM240780337 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM240780337 | ||
003 | DE-627 | ||
005 | 20231224122828.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231224s2014 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1371/journal.pone.0104524 |2 doi | |
028 | 5 | 2 | |a pubmed24n0802.xml |
035 | |a (DE-627)NLM240780337 | ||
035 | |a (NLM)25101850 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Bozue, Joel |e verfasserin |4 aut | |
245 | 1 | 2 | |a A Yersinia pestis tat mutant is attenuated in bubonic and small-aerosol pneumonic challenge models of infection but not as attenuated by intranasal challenge |
264 | 1 | |c 2014 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 30.11.2015 | ||
500 | |a Date Revised 21.10.2021 | ||
500 | |a published: Electronic-eCollection | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Bacterial proteins destined for the Tat pathway are folded before crossing the inner membrane and are typically identified by an N-terminal signal peptide containing a twin arginine motif. Translocation by the Tat pathway is dependent on the products of genes which encode proteins possessing the binding site of the signal peptide and mediating the actual translocation event. In the fully virulent CO92 strain of Yersinia pestis, the tatA gene was deleted. The mutant was assayed for loss of virulence through various in vitro and in vivo assays. Deletion of the tatA gene resulted in several consequences for the mutant as compared to wild-type. Cell morphology of the mutant bacteria was altered and demonstrated a more elongated form. In addition, while cultures of the mutant strain were able to produce a biofilm, we observed a loss of adhesion of the mutant biofilm structure compared to the biofilm produced by the wild-type strain. Immuno-electron microscopy revealed a partial disruption of the F1 antigen on the surface of the mutant. The virulence of the ΔtatA mutant was assessed in various murine models of plague. The mutant was severely attenuated in the bubonic model with full virulence restored by complementation with the native gene. After small-particle aerosol challenge in a pneumonic model of infection, the mutant was also shown to be attenuated. In contrast, when mice were challenged intranasally with the mutant, very little difference in the LD50 was observed between wild-type and mutant strains. However, an increased time-to-death and delay in bacterial dissemination was observed in mice infected with the ΔtatA mutant as compared to the parent strain. Collectively, these findings demonstrate an essential role for the Tat pathway in the virulence of Y. pestis in bubonic and small-aerosol pneumonic infection but less important role for intranasal challenge | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
650 | 7 | |a Aerosols |2 NLM | |
650 | 7 | |a Bacterial Proteins |2 NLM | |
650 | 7 | |a Membrane Transport Proteins |2 NLM | |
700 | 1 | |a Cote, Christopher K |e verfasserin |4 aut | |
700 | 1 | |a Chance, Taylor |e verfasserin |4 aut | |
700 | 1 | |a Kugelman, Jeffrey |e verfasserin |4 aut | |
700 | 1 | |a Kern, Steven J |e verfasserin |4 aut | |
700 | 1 | |a Kijek, Todd K |e verfasserin |4 aut | |
700 | 1 | |a Jenkins, Amy |e verfasserin |4 aut | |
700 | 1 | |a Mou, Sherry |e verfasserin |4 aut | |
700 | 1 | |a Moody, Krishna |e verfasserin |4 aut | |
700 | 1 | |a Fritz, David |e verfasserin |4 aut | |
700 | 1 | |a Robinson, Camenzind G |e verfasserin |4 aut | |
700 | 1 | |a Bell, Todd |e verfasserin |4 aut | |
700 | 1 | |a Worsham, Patricia |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t PloS one |d 2006 |g 9(2014), 8 vom: 06., Seite e104524 |w (DE-627)NLM167327399 |x 1932-6203 |7 nnns |
773 | 1 | 8 | |g volume:9 |g year:2014 |g number:8 |g day:06 |g pages:e104524 |
856 | 4 | 0 | |u http://dx.doi.org/10.1371/journal.pone.0104524 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 9 |j 2014 |e 8 |b 06 |h e104524 |