Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligands
© 2014 by The American Society for Biochemistry and Molecular Biology, Inc..
PDZ domain proteins control multiple cellular functions by governing assembly of protein complexes. It remains unknown why individual PDZ domains can bind the extreme C terminus of very diverse binding partners and maintain selectivity. By employing NMR spectroscopy, together with molecular modeling, mutational analysis, and fluorescent polarization binding experiments, we identify here three structural mechanisms explaining why the PDZ domain of PICK1 selectively binds >30 receptors, transporters, and kinases. Class II ligands, including the dopamine transporter, adopt a canonical binding mode with promiscuity obtained via differential packing in the binding groove. Class I ligands, such as protein kinase Cα, depend on residues upstream from the canonical binding sequence that are likely to interact with flexible loop residues of the PDZ domain. Finally, we obtain evidence that the unconventional ligand ASIC1a has a dual binding mode involving a canonical insertion and a noncanonical internal insertion with the two C-terminal residues forming interactions outside the groove. Together with an evolutionary analysis, the data show how unconventional binding modes might evolve for a protein recognition domain to expand the repertoire of functionally important interactions.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2014 |
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Erschienen: |
2014 |
Enthalten in: |
Zur Gesamtaufnahme - volume:289 |
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Enthalten in: |
The Journal of biological chemistry - 289(2014), 36 vom: 05. Sept., Seite 25327-40 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Erlendsson, Simon [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 28.01.2015 Date Revised 09.03.2022 published: Print-Electronic PDB: 2LUI Citation Status MEDLINE |
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doi: |
10.1074/jbc.M114.548743 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM24005556X |
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245 | 1 | 0 | |a Protein interacting with C-kinase 1 (PICK1) binding promiscuity relies on unconventional PSD-95/discs-large/ZO-1 homology (PDZ) binding modes for nonclass II PDZ ligands |
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500 | |a published: Print-Electronic | ||
500 | |a PDB: 2LUI | ||
500 | |a Citation Status MEDLINE | ||
520 | |a © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. | ||
520 | |a PDZ domain proteins control multiple cellular functions by governing assembly of protein complexes. It remains unknown why individual PDZ domains can bind the extreme C terminus of very diverse binding partners and maintain selectivity. By employing NMR spectroscopy, together with molecular modeling, mutational analysis, and fluorescent polarization binding experiments, we identify here three structural mechanisms explaining why the PDZ domain of PICK1 selectively binds >30 receptors, transporters, and kinases. Class II ligands, including the dopamine transporter, adopt a canonical binding mode with promiscuity obtained via differential packing in the binding groove. Class I ligands, such as protein kinase Cα, depend on residues upstream from the canonical binding sequence that are likely to interact with flexible loop residues of the PDZ domain. Finally, we obtain evidence that the unconventional ligand ASIC1a has a dual binding mode involving a canonical insertion and a noncanonical internal insertion with the two C-terminal residues forming interactions outside the groove. Together with an evolutionary analysis, the data show how unconventional binding modes might evolve for a protein recognition domain to expand the repertoire of functionally important interactions | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a Nuclear Magnetic Resonance (NMR) | |
650 | 4 | |a PDZ Domains | |
650 | 4 | |a Protein Structure | |
650 | 4 | |a Protein-Protein Interaction | |
650 | 4 | |a Scaffold Protein | |
650 | 4 | |a Signal Transduction | |
650 | 4 | |a Trafficking | |
650 | 7 | |a Carrier Proteins |2 NLM | |
650 | 7 | |a Ligands |2 NLM | |
650 | 7 | |a Nuclear Proteins |2 NLM | |
650 | 7 | |a PICk1 protein, human |2 NLM | |
650 | 7 | |a Peptides |2 NLM | |
650 | 7 | |a Protein Kinase C-alpha |2 NLM | |
650 | 7 | |a EC 2.7.11.13 |2 NLM | |
700 | 1 | |a Rathje, Mette |e verfasserin |4 aut | |
700 | 1 | |a Heidarsson, Pétur O |e verfasserin |4 aut | |
700 | 1 | |a Poulsen, Flemming M |e verfasserin |4 aut | |
700 | 1 | |a Madsen, Kenneth L |e verfasserin |4 aut | |
700 | 1 | |a Teilum, Kaare |e verfasserin |4 aut | |
700 | 1 | |a Gether, Ulrik |e verfasserin |4 aut | |
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