Phosphatase 1 nuclear targeting subunit is an essential regulator of M-phase entry, maintenance, and exit
© 2014 by The American Society for Biochemistry and Molecular Biology, Inc..
Mitotic progression is regulated largely through dynamic and reversible protein phosphorylation that is modulated by opposing actions of protein kinases and phosphatases. In this study, we show that phosphatase 1 nuclear targeting subunit (Pnuts) functions as a master regulator of mitosis by modulating protein phosphatase 1 (PP1). Overexpression of Pnuts in Xenopus egg extracts inhibited both mitotic and meiotic exit. Immunodepletion of Pnuts from egg extracts revealed its essential functions in mitotic entry and maintenance. The level of Pnuts oscillates during the cell cycle and peaks in mitosis. Pnuts destruction during M-phase exit is mediated by the anaphase-promoting complex/cyclosome (APC/C)-targeted ubiquitination and proteolysis, and conserved destruction motifs of Pnuts. Disruption of Pnuts degradation delayed M-phase exit, suggesting it as an important mechanism to permit M-phase exit.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:289 |
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| Enthalten in: |
The Journal of biological chemistry - 289(2014), 34 vom: 22. Aug., Seite 23745-52 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fisher, Laura A [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.12.2014 Date Revised 21.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1074/jbc.M114.572149 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM239880935 |
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| 245 | 1 | 0 | |a Phosphatase 1 nuclear targeting subunit is an essential regulator of M-phase entry, maintenance, and exit |
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| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. | ||
| 520 | |a Mitotic progression is regulated largely through dynamic and reversible protein phosphorylation that is modulated by opposing actions of protein kinases and phosphatases. In this study, we show that phosphatase 1 nuclear targeting subunit (Pnuts) functions as a master regulator of mitosis by modulating protein phosphatase 1 (PP1). Overexpression of Pnuts in Xenopus egg extracts inhibited both mitotic and meiotic exit. Immunodepletion of Pnuts from egg extracts revealed its essential functions in mitotic entry and maintenance. The level of Pnuts oscillates during the cell cycle and peaks in mitosis. Pnuts destruction during M-phase exit is mediated by the anaphase-promoting complex/cyclosome (APC/C)-targeted ubiquitination and proteolysis, and conserved destruction motifs of Pnuts. Disruption of Pnuts degradation delayed M-phase exit, suggesting it as an important mechanism to permit M-phase exit | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Cell Cycle | |
| 650 | 4 | |a Mitosis | |
| 650 | 4 | |a Phosphatase | |
| 650 | 4 | |a Phosphoprotein Phosphatase 1 (PP1) | |
| 650 | 4 | |a Pnuts | |
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| 700 | 1 | |a Wang, Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Lan |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Aimin |e verfasserin |4 aut | |
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