Aberrant mucosal lymphocyte number and subsets in the colon of post-infectious irritable bowel syndrome patients
BACKGROUND: Irritable bowel syndrome (IBS) is characterized by chronic abdominal symptoms such as pain, discomfort, and altered bowel habits. A subset of IBS patients, denoted as post-infectious IBS (PI-IBS) patients, develop symptoms after an enteric infection. Distinct abnormalities in the gut mucosa, including mucosal inflammation, have been proposed to contribute to or be the cause of PI-IBS. This study investigated lymphocyte subsets in PI-IBS patients compared to healthy controls.
MATERIALS AND METHODS: Ten PI-IBS patients and nine healthy controls participated. All PI-IBS patients met the Rome III diagnostic criteria for IBS and reported sustained symptoms at least 1 year after an episode of acute gastroenteritis. Intraepithelial lymphocytes and lamina propria lymphocytes (LPLs), isolated from mucosal tissue samples, were stained and analyzed for a comprehensive set of cell markers using flow cytometry.
RESULTS: The number of LPLs in PI-IBS was significantly increased compared to those in healthy controls (p < 0.05). PI-IBS patients showed significantly increased proportions of CD45RO(+) CD4(+) activated/memory T cells (p < 0.05) and double-positive CD4(+) CD8(+) cells (p < 0.05), respectively, in the lamina propria. The number of CD19(+) LPLs was decreased in PI-IBS patients compared to healthy controls (p < 0.001).
CONCLUSION: This study presents new evidence that PI-IBS is associated with a sustained aberrant mucosal immune response and support future studies of anti-inflammatory or immune-modulating treatments in these patients.
| Errataetall: |
CommentIn: Cell Mol Immunol. 2021 Jul;18(7):1620-1621. - PMID 33479415 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:49 |
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| Enthalten in: |
Scandinavian journal of gastroenterology - 49(2014), 9 vom: 16. Sept., Seite 1068-75 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sundin, Johanna [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.05.2015 Date Revised 16.11.2021 published: Print-Electronic CommentIn: Cell Mol Immunol. 2021 Jul;18(7):1620-1621. - PMID 33479415 Citation Status MEDLINE |
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| doi: |
10.3109/00365521.2014.926982 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM239094425 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a CommentIn: Cell Mol Immunol. 2021 Jul;18(7):1620-1621. - PMID 33479415 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Irritable bowel syndrome (IBS) is characterized by chronic abdominal symptoms such as pain, discomfort, and altered bowel habits. A subset of IBS patients, denoted as post-infectious IBS (PI-IBS) patients, develop symptoms after an enteric infection. Distinct abnormalities in the gut mucosa, including mucosal inflammation, have been proposed to contribute to or be the cause of PI-IBS. This study investigated lymphocyte subsets in PI-IBS patients compared to healthy controls | ||
| 520 | |a MATERIALS AND METHODS: Ten PI-IBS patients and nine healthy controls participated. All PI-IBS patients met the Rome III diagnostic criteria for IBS and reported sustained symptoms at least 1 year after an episode of acute gastroenteritis. Intraepithelial lymphocytes and lamina propria lymphocytes (LPLs), isolated from mucosal tissue samples, were stained and analyzed for a comprehensive set of cell markers using flow cytometry | ||
| 520 | |a RESULTS: The number of LPLs in PI-IBS was significantly increased compared to those in healthy controls (p < 0.05). PI-IBS patients showed significantly increased proportions of CD45RO(+) CD4(+) activated/memory T cells (p < 0.05) and double-positive CD4(+) CD8(+) cells (p < 0.05), respectively, in the lamina propria. The number of CD19(+) LPLs was decreased in PI-IBS patients compared to healthy controls (p < 0.001) | ||
| 520 | |a CONCLUSION: This study presents new evidence that PI-IBS is associated with a sustained aberrant mucosal immune response and support future studies of anti-inflammatory or immune-modulating treatments in these patients | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a cell biology | |
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| 700 | 1 | |a Kumawat, Ashok K |e verfasserin |4 aut | |
| 700 | 1 | |a Hultgren-Hörnquist, Elisabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Brummer, Robert J |e verfasserin |4 aut | |
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