Sample treatment platform using nanoparticles to determine salinomycin in flesh and meat
Copyright © 2014 Elsevier Ltd. All rights reserved..
In this work, we developed a sensitive and selective analytical method of determining salinomycin in flesh and meat using a lab-built laser induced fluorescence microscope (LIFM) with nanoparticles. Two types of nanoparticles, Cy5 doped core-shell silica nanoparticles as a probe and magnetic nanoparticles (MNPs) to extract the target antibiotic from the treated sample, were synthesized and modified for chemical and enzymatic binding. After optimization, the newly developed method was applied to ham, chicken, and meat samples for the quantitative determination of salinomycin. The limits of detection (LODs) obtained from the calibration curve were 48-590 pg/mL, which was about 100-fold lower than that of the ELISA method.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:160 |
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| Enthalten in: |
Food chemistry - 160(2014) vom: 01. Okt., Seite 112-7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Park, Jihyun [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 04.11.2015 Date Revised 02.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2014.03.047 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In this work, we developed a sensitive and selective analytical method of determining salinomycin in flesh and meat using a lab-built laser induced fluorescence microscope (LIFM) with nanoparticles. Two types of nanoparticles, Cy5 doped core-shell silica nanoparticles as a probe and magnetic nanoparticles (MNPs) to extract the target antibiotic from the treated sample, were synthesized and modified for chemical and enzymatic binding. After optimization, the newly developed method was applied to ham, chicken, and meat samples for the quantitative determination of salinomycin. The limits of detection (LODs) obtained from the calibration curve were 48-590 pg/mL, which was about 100-fold lower than that of the ELISA method | ||
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