Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases
Conflicting data are reported on pro- or anti-inflammatory activity of bovine lactoferrin (bLf) in different cell models as phagocytes or epithelial cell lines infected by bacteria. Here we evaluated the bLf effect on epithelial models mimicking two human pathologies characterized by inflammation and infection with specific bacterial species. Primary bronchial epithelium from a cystic fibrosis (CF) patient and differentiated intestinal epithelial cells were infected with Pseudomonas aeruginosa LESB58 isolated from a CF patient and Adherent-Invasive Escherichia coli LF82 isolated from a Crohn's disease patient. Surprisingly, bLf significantly reduced the intracellular bacterial survival, but differently modulated the inflammatory response. These data lead us to hypothesize that bLf differentially acts depending on the epithelial model and infecting pathogen. To verify this hypothesis, we explored whether bLf could modulate ferroportin (Fpn), the only known cellular iron exporter from cells, that, by lowering the intracellular iron level, determines a non permissive environment for intracellular pathogens. Here, for the first time, we describe the bLf ability to up-regulate Fpn protein in infected epithelial models. Our data suggest that the mechanism underlying the bLf modulating activity on inflammatory response in epithelial cells is complex and the bLf involvement in modulating cellular iron homeostasis should be taken into account.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:27 |
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| Enthalten in: |
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine - 27(2014), 5 vom: 07. Okt., Seite 843-56 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Frioni, Alessandra [VerfasserIn] |
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| Links: |
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| Themen: |
Antimicrobial Cationic Peptides |
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| Anmerkungen: |
Date Completed 24.07.2015 Date Revised 05.09.2014 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s10534-014-9740-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 100 | 1 | |a Frioni, Alessandra |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lactoferrin differently modulates the inflammatory response in epithelial models mimicking human inflammatory and infectious diseases |
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| 520 | |a Conflicting data are reported on pro- or anti-inflammatory activity of bovine lactoferrin (bLf) in different cell models as phagocytes or epithelial cell lines infected by bacteria. Here we evaluated the bLf effect on epithelial models mimicking two human pathologies characterized by inflammation and infection with specific bacterial species. Primary bronchial epithelium from a cystic fibrosis (CF) patient and differentiated intestinal epithelial cells were infected with Pseudomonas aeruginosa LESB58 isolated from a CF patient and Adherent-Invasive Escherichia coli LF82 isolated from a Crohn's disease patient. Surprisingly, bLf significantly reduced the intracellular bacterial survival, but differently modulated the inflammatory response. These data lead us to hypothesize that bLf differentially acts depending on the epithelial model and infecting pathogen. To verify this hypothesis, we explored whether bLf could modulate ferroportin (Fpn), the only known cellular iron exporter from cells, that, by lowering the intracellular iron level, determines a non permissive environment for intracellular pathogens. Here, for the first time, we describe the bLf ability to up-regulate Fpn protein in infected epithelial models. Our data suggest that the mechanism underlying the bLf modulating activity on inflammatory response in epithelial cells is complex and the bLf involvement in modulating cellular iron homeostasis should be taken into account | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Conte, Maria Pia |e verfasserin |4 aut | |
| 700 | 1 | |a Cutone, Antimo |e verfasserin |4 aut | |
| 700 | 1 | |a Longhi, Catia |e verfasserin |4 aut | |
| 700 | 1 | |a Musci, Giovanni |e verfasserin |4 aut | |
| 700 | 1 | |a di Patti, Maria Carmela Bonaccorsi |e verfasserin |4 aut | |
| 700 | 1 | |a Natalizi, Tiziana |e verfasserin |4 aut | |
| 700 | 1 | |a Marazzato, Massimiliano |e verfasserin |4 aut | |
| 700 | 1 | |a Lepanto, Maria Stefania |e verfasserin |4 aut | |
| 700 | 1 | |a Puddu, Patrizia |e verfasserin |4 aut | |
| 700 | 1 | |a Paesano, Rosalba |e verfasserin |4 aut | |
| 700 | 1 | |a Valenti, Piera |e verfasserin |4 aut | |
| 700 | 1 | |a Berlutti, Francesca |e verfasserin |4 aut | |
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