New bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings
Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved..
INTRODUCTION: The aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings.
METHODS: Samples from the root canals of 21 German patients were taken using 3 sequential sterile paper points. In the case of a root canal filling, gutta-percha was removed with sterile files, and samples were taken using sterile paper points. The samples were plated, and microorganisms were then isolated and identified morphologically by biochemical analysis and sequencing the 16S rRNA genes of isolated microorganisms.
RESULTS: In 12 of 21 root canals, 33 different species could be isolated. Six (50%) of the cases with isolated microorganisms were primary, and 6 (50%) cases were endodontic infections associated with root-filled teeth. Twelve of the isolated species were facultative anaerobic and 21 obligate anaerobic. Monomicrobial infections were found for Enterococcus faecalis and Actinomyces viscosus. E. faecalis was most frequently isolated in secondary endodontic infections (33%). Moraxella osloensis was isolated from a secondary endodontic infection that had an insufficient root canal filling accompanied by a mild sensation of pain. A new bacterial composition compromising Atopobium rimae, Anaerococcus prevotii, Pseudoramibacter alactolyticus, Dialister invisus, and Fusobacterium nucleatum was recovered from teeth with chronic apical abscesses.
CONCLUSIONS: New bacterial combinations were found and correlated to clinical and radiographic findings, particularly to chronic apical abscesses. M. osloensis was detected in root canals for the second time and only in German patients.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2014 |
|---|---|
| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
|---|---|
| Enthalten in: |
Journal of endodontics - 40(2014), 5 vom: 26. Mai, Seite 670-7 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Tennert, Christian [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Apical periodontitis |
|---|
| Anmerkungen: |
Date Completed 26.07.2016 Date Revised 09.04.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.joen.2013.10.005 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM237699419 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM237699419 | ||
| 003 | DE-627 | ||
| 005 | 20231224112218.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.joen.2013.10.005 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0792.xml |
| 035 | |a (DE-627)NLM237699419 | ||
| 035 | |a (NLM)24767562 | ||
| 035 | |a (PII)S0099-2399(13)00901-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Tennert, Christian |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a New bacterial composition in primary and persistent/secondary endodontic infections with respect to clinical and radiographic findings |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 26.07.2016 | ||
| 500 | |a Date Revised 09.04.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2014 American Association of Endodontists. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a INTRODUCTION: The aim of the present study was to analyze the microbiota of primary and secondary/persistent endodontic infections of patients undergoing endodontic treatment with respect to clinical and radiographic findings | ||
| 520 | |a METHODS: Samples from the root canals of 21 German patients were taken using 3 sequential sterile paper points. In the case of a root canal filling, gutta-percha was removed with sterile files, and samples were taken using sterile paper points. The samples were plated, and microorganisms were then isolated and identified morphologically by biochemical analysis and sequencing the 16S rRNA genes of isolated microorganisms | ||
| 520 | |a RESULTS: In 12 of 21 root canals, 33 different species could be isolated. Six (50%) of the cases with isolated microorganisms were primary, and 6 (50%) cases were endodontic infections associated with root-filled teeth. Twelve of the isolated species were facultative anaerobic and 21 obligate anaerobic. Monomicrobial infections were found for Enterococcus faecalis and Actinomyces viscosus. E. faecalis was most frequently isolated in secondary endodontic infections (33%). Moraxella osloensis was isolated from a secondary endodontic infection that had an insufficient root canal filling accompanied by a mild sensation of pain. A new bacterial composition compromising Atopobium rimae, Anaerococcus prevotii, Pseudoramibacter alactolyticus, Dialister invisus, and Fusobacterium nucleatum was recovered from teeth with chronic apical abscesses | ||
| 520 | |a CONCLUSIONS: New bacterial combinations were found and correlated to clinical and radiographic findings, particularly to chronic apical abscesses. M. osloensis was detected in root canals for the second time and only in German patients | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Apical periodontitis | |
| 650 | 4 | |a dental trauma | |
| 650 | 4 | |a endodontic infection | |
| 650 | 4 | |a endodontic microorganisms | |
| 650 | 4 | |a root canal treatment | |
| 650 | 7 | |a RNA, Bacterial |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
| 700 | 1 | |a Fuhrmann, Maximilian |e verfasserin |4 aut | |
| 700 | 1 | |a Wittmer, Annette |e verfasserin |4 aut | |
| 700 | 1 | |a Karygianni, Lamprini |e verfasserin |4 aut | |
| 700 | 1 | |a Altenburger, Markus J |e verfasserin |4 aut | |
| 700 | 1 | |a Pelz, Klaus |e verfasserin |4 aut | |
| 700 | 1 | |a Hellwig, Elmar |e verfasserin |4 aut | |
| 700 | 1 | |a Al-Ahmad, Ali |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of endodontics |d 1975 |g 40(2014), 5 vom: 26. Mai, Seite 670-7 |w (DE-627)NLM000185019 |x 1878-3554 |7 nnns |
| 773 | 1 | 8 | |g volume:40 |g year:2014 |g number:5 |g day:26 |g month:05 |g pages:670-7 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.joen.2013.10.005 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 40 |j 2014 |e 5 |b 26 |c 05 |h 670-7 | ||