Three-dimensional visualization of perlecan-rich neoplastic stroma induced concurrently with the invasion of oral squamous cell carcinoma
© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd..
BACKGROUND: We have demonstrated the induction of perlecan-rich stroma of oral squamous cell carcinoma (SCC) on and after its start of invasion. However, it remains unknown how such a neoplastic stroma is actually arranged in tumor tissues.
METHODS: To this end, tissue microarray samples, in which keratin and perlecan were contrastively labeled by immunohistochemistry, were three-dimensionally analyzed using digital images and image analysis software to demonstrate the relationship between SCC foci and the perlecan-positive stromal space or that between carcinoma in situ (CIS) and invasive SCC foci.
RESULTS: The three-dimensional (3D) reconstruction demonstrated three kinds of perlecan profiles for inside (I) and outside (O) areas of the carcinoma cell focus: mode 1, I(+)/O(-) ; mode 2, I(+)/O(+) ; and mode 3, I(-)/O(+). Mode 1 was seen in CIS as well as SCC tumor massifs in the surface part. Mode 2 was seen in small SCC foci, which seemed isolated in 2D sections but were mostly continuous with the tumor massif in 3D reconstructions. Mode 3 was limited to small SCC foci, which were truly segregated from the tumor massif.
CONCLUSIONS: The results indicated that the 2D SCC focus isolation could not be regarded as invasion but that the SCC foci surrounded by perlecan-positive stroma (modes 2 and 3) could be regarded as a more objective measure for invasion of SCC. This is the first 3D tissue-level demonstration of the neoplastic stroma space induced with oral SCC invasion, the presence of which we have predicted based on our previous 2D and tissue culture evidence.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:43 |
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| Enthalten in: |
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology - 43(2014), 8 vom: 27. Sept., Seite 627-36 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Maruyama, Satoshi [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 19.06.2015 Date Revised 13.12.2017 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/jop.12184 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM237035510 |
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| 100 | 1 | |a Maruyama, Satoshi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Three-dimensional visualization of perlecan-rich neoplastic stroma induced concurrently with the invasion of oral squamous cell carcinoma |
| 264 | 1 | |c 2014 | |
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| 500 | |a Date Completed 19.06.2015 | ||
| 500 | |a Date Revised 13.12.2017 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | ||
| 520 | |a BACKGROUND: We have demonstrated the induction of perlecan-rich stroma of oral squamous cell carcinoma (SCC) on and after its start of invasion. However, it remains unknown how such a neoplastic stroma is actually arranged in tumor tissues | ||
| 520 | |a METHODS: To this end, tissue microarray samples, in which keratin and perlecan were contrastively labeled by immunohistochemistry, were three-dimensionally analyzed using digital images and image analysis software to demonstrate the relationship between SCC foci and the perlecan-positive stromal space or that between carcinoma in situ (CIS) and invasive SCC foci | ||
| 520 | |a RESULTS: The three-dimensional (3D) reconstruction demonstrated three kinds of perlecan profiles for inside (I) and outside (O) areas of the carcinoma cell focus: mode 1, I(+)/O(-) ; mode 2, I(+)/O(+) ; and mode 3, I(-)/O(+). Mode 1 was seen in CIS as well as SCC tumor massifs in the surface part. Mode 2 was seen in small SCC foci, which seemed isolated in 2D sections but were mostly continuous with the tumor massif in 3D reconstructions. Mode 3 was limited to small SCC foci, which were truly segregated from the tumor massif | ||
| 520 | |a CONCLUSIONS: The results indicated that the 2D SCC focus isolation could not be regarded as invasion but that the SCC foci surrounded by perlecan-positive stroma (modes 2 and 3) could be regarded as a more objective measure for invasion of SCC. This is the first 3D tissue-level demonstration of the neoplastic stroma space induced with oral SCC invasion, the presence of which we have predicted based on our previous 2D and tissue culture evidence | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a invasion judgment | |
| 650 | 4 | |a neoplastic stroma | |
| 650 | 4 | |a oral squamous cell carcinoma | |
| 650 | 4 | |a perlecan | |
| 650 | 4 | |a three-dimensional visualization | |
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| 700 | 1 | |a Shimazu, Yoshihito |e verfasserin |4 aut | |
| 700 | 1 | |a Kudo, Tomoo |e verfasserin |4 aut | |
| 700 | 1 | |a Sato, Kaori |e verfasserin |4 aut | |
| 700 | 1 | |a Yamazaki, Manabu |e verfasserin |4 aut | |
| 700 | 1 | |a Abé, Tatsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Babkair, Hamzah |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Aoba, Takaaki |e verfasserin |4 aut | |
| 700 | 1 | |a Saku, Takashi |e verfasserin |4 aut | |
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