The mixture of "ecstasy" and its metabolites impairs mitochondrial fusion/fission equilibrium and trafficking in hippocampal neurons, at in vivo relevant concentrations
3,4-Methylenedioxymethamphetamine (MDMA; "ecstasy") is a potentially neurotoxic recreational drug of abuse. Though the mechanisms involved are still not completely understood, formation of reactive metabolites and mitochondrial dysfunction contribute to MDMA-related neurotoxicity. Neuronal mitochondrial trafficking, and their targeting to synapses, is essential for proper neuronal function and survival, rendering neurons particularly vulnerable to mitochondrial dysfunction. Indeed, MDMA-associated disruption of Ca(2+) homeostasis and ATP depletion have been described in neurons, thus suggesting possible MDMA interference on mitochondrial dynamics. In this study, we performed real-time functional experiments of mitochondrial trafficking to explore the role of in situ mitochondrial dysfunction in MDMA's neurotoxic actions. We show that the mixture of MDMA and six of its major in vivo metabolites, each compound at 10μM, impaired mitochondrial trafficking and increased the fragmentation of axonal mitochondria in cultured hippocampal neurons. Furthermore, the overexpression of mitofusin 2 (Mfn2) or dynamin-related protein 1 (Drp1) K38A constructs almost completely rescued the trafficking deficits caused by this mixture. Finally, in hippocampal neurons overexpressing a Mfn2 mutant, Mfn2 R94Q, with impaired fusion and transport properties, it was confirmed that a dysregulation of mitochondrial fission/fusion events greatly contributed to the reported trafficking phenotype. In conclusion, our study demonstrated, for the first time, that the mixture of MDMA and its metabolites, at concentrations relevant to the in vivo scenario, impaired mitochondrial trafficking and increased mitochondrial fragmentation in hippocampal neurons, thus providing a new insight in the context of "ecstasy"-induced neuronal injury.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:139 |
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| Enthalten in: |
Toxicological sciences : an official journal of the Society of Toxicology - 139(2014), 2 vom: 05. Juni, Seite 407-20 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Barbosa, Daniel José [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 29.12.2014 Date Revised 12.05.2014 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1093/toxsci/kfu042 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM236094378 |
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| 100 | 1 | |a Barbosa, Daniel José |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The mixture of "ecstasy" and its metabolites impairs mitochondrial fusion/fission equilibrium and trafficking in hippocampal neurons, at in vivo relevant concentrations |
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| 520 | |a 3,4-Methylenedioxymethamphetamine (MDMA; "ecstasy") is a potentially neurotoxic recreational drug of abuse. Though the mechanisms involved are still not completely understood, formation of reactive metabolites and mitochondrial dysfunction contribute to MDMA-related neurotoxicity. Neuronal mitochondrial trafficking, and their targeting to synapses, is essential for proper neuronal function and survival, rendering neurons particularly vulnerable to mitochondrial dysfunction. Indeed, MDMA-associated disruption of Ca(2+) homeostasis and ATP depletion have been described in neurons, thus suggesting possible MDMA interference on mitochondrial dynamics. In this study, we performed real-time functional experiments of mitochondrial trafficking to explore the role of in situ mitochondrial dysfunction in MDMA's neurotoxic actions. We show that the mixture of MDMA and six of its major in vivo metabolites, each compound at 10μM, impaired mitochondrial trafficking and increased the fragmentation of axonal mitochondria in cultured hippocampal neurons. Furthermore, the overexpression of mitofusin 2 (Mfn2) or dynamin-related protein 1 (Drp1) K38A constructs almost completely rescued the trafficking deficits caused by this mixture. Finally, in hippocampal neurons overexpressing a Mfn2 mutant, Mfn2 R94Q, with impaired fusion and transport properties, it was confirmed that a dysregulation of mitochondrial fission/fusion events greatly contributed to the reported trafficking phenotype. In conclusion, our study demonstrated, for the first time, that the mixture of MDMA and its metabolites, at concentrations relevant to the in vivo scenario, impaired mitochondrial trafficking and increased mitochondrial fragmentation in hippocampal neurons, thus providing a new insight in the context of "ecstasy"-induced neuronal injury | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a 3,4-methylenedioxymethamphetamine (MDMA; “ecstasy”) | |
| 650 | 4 | |a MDMA's metabolites | |
| 650 | 4 | |a mitochondrial dysfunction | |
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| 650 | 4 | |a mitochondrial trafficking | |
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| 700 | 1 | |a Mirra, Serena |e verfasserin |4 aut | |
| 700 | 1 | |a Quevedo, Martí |e verfasserin |4 aut | |
| 700 | 1 | |a de Barreda, Elena Goméz |e verfasserin |4 aut | |
| 700 | 1 | |a Àvila, Jesús |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Luísa Maria |e verfasserin |4 aut | |
| 700 | 1 | |a Branco, Paula Sério |e verfasserin |4 aut | |
| 700 | 1 | |a Fernandes, Eduarda |e verfasserin |4 aut | |
| 700 | 1 | |a Lourdes Bastos, Maria de |e verfasserin |4 aut | |
| 700 | 1 | |a Capela, João Paulo |e verfasserin |4 aut | |
| 700 | 1 | |a Soriano, Eduardo |e verfasserin |4 aut | |
| 700 | 1 | |a Carvalho, Félix |e verfasserin |4 aut | |
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