Genetic characterization analysis on epidemic rubella virus strains isolated in Liaoning from 2007 to 2012
To analyze the genetic characterization of epidemic rubella virus strains isolated in Liaoning from 2007-2012, a total of 145 rubella virus strains were isolated using Vero/Slam cell line from the patients' throat swabs during rubella outbreaks and sporadics cases in Liaoning Province from 2007 to 2012. Fragments of 945 nucleotides containing 1E gene from 145 rubella virus isolates were amplified by RT-PCR, the PCR products were sequenced and analyzed. Based on the 739 nucleotides of 1E gene, the phylogenetic trees were constructed with 32 WHO rubella reference strains of 13 genotypes downloaded from GenBank and 145 rubella virus strains. The results showed that the 145 rubella virus strains in 2007 -2012 belonged to genotype 1E, nucleotide acids and amino acids similarities were 97.2%-100.0% and 97.6%-100.0%, respectively. Compared to the 1E reference strains(Rvi/ Dezhou.CHN/02, RVi/MYS/01), the nucleotide acids and amino acids similarities were 96.6%-99.2% and 98.2%-100.0%, respectively except for one amino acid change (Val246-Ala246) of RVi/Shenyang. Liaoning. CHN/13.11/13, and Asp262-Asn262 of RVi/Shenyang. Liaoning. CHN/13.11/4 and RVi/Liaoyang. Liaoning. CHN/26. 11/2. there had no change found in the important antigenic epitope sites, the hemagglutination inhibition and neutralization epitopes of the other rubella viruses. All the 145 strains isolated had the same amino acid change (Leu338--Phe338) in E1 protein. These findings suggested that genotype 1E of rubella virus was the predominant genotype in Liaoning province. the rubella prevailed in recent six years was mainly caused by rubella viruses genotype 1E with multi-transmission routes.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Bing du xue bao = Chinese journal of virology - 29(2013), 6 vom: 01. Nov., Seite 589-95 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Wang, Yan [VerfasserIn] |
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| Themen: |
English Abstract |
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| Anmerkungen: |
Date Completed 01.04.2014 Date Revised 21.10.2016 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM235383031 |
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| 100 | 1 | |a Wang, Yan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genetic characterization analysis on epidemic rubella virus strains isolated in Liaoning from 2007 to 2012 |
| 264 | 1 | |c 2013 | |
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| 500 | |a Date Completed 01.04.2014 | ||
| 500 | |a Date Revised 21.10.2016 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a To analyze the genetic characterization of epidemic rubella virus strains isolated in Liaoning from 2007-2012, a total of 145 rubella virus strains were isolated using Vero/Slam cell line from the patients' throat swabs during rubella outbreaks and sporadics cases in Liaoning Province from 2007 to 2012. Fragments of 945 nucleotides containing 1E gene from 145 rubella virus isolates were amplified by RT-PCR, the PCR products were sequenced and analyzed. Based on the 739 nucleotides of 1E gene, the phylogenetic trees were constructed with 32 WHO rubella reference strains of 13 genotypes downloaded from GenBank and 145 rubella virus strains. The results showed that the 145 rubella virus strains in 2007 -2012 belonged to genotype 1E, nucleotide acids and amino acids similarities were 97.2%-100.0% and 97.6%-100.0%, respectively. Compared to the 1E reference strains(Rvi/ Dezhou.CHN/02, RVi/MYS/01), the nucleotide acids and amino acids similarities were 96.6%-99.2% and 98.2%-100.0%, respectively except for one amino acid change (Val246-Ala246) of RVi/Shenyang. Liaoning. CHN/13.11/13, and Asp262-Asn262 of RVi/Shenyang. Liaoning. CHN/13.11/4 and RVi/Liaoyang. Liaoning. CHN/26. 11/2. there had no change found in the important antigenic epitope sites, the hemagglutination inhibition and neutralization epitopes of the other rubella viruses. All the 145 strains isolated had the same amino acid change (Leu338--Phe338) in E1 protein. These findings suggested that genotype 1E of rubella virus was the predominant genotype in Liaoning province. the rubella prevailed in recent six years was mainly caused by rubella viruses genotype 1E with multi-transmission routes | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Viral Envelope Proteins |2 NLM | |
| 700 | 1 | |a Ma, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Xiao-Ting |e verfasserin |4 aut | |
| 700 | 1 | |a Fan, Xue-Song |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Sui, Dan |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Feng-Tong |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Bai-Ling |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Guang-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ji-Jian |e verfasserin |4 aut | |
| 700 | 1 | |a Han, Yue |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Jun-Qiao |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Zhuo |e verfasserin |4 aut | |
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