The effect of RABEX-5 downregulation on the chemosensitivity of human breast cancer cells
OBJECTIVE: To investigate the changes in sensitivity to anthracycline and taxanes of human breast cancer cells after RABEX-5 downregulated.
METHODS: By constructing a lentiviral vector for RNA interference (RNAi) of RABEX-5 gene and transfected into human breast cancer cell line MCF-7 to silence the express of RABEX-5. Real-time PCR and Western blot were used to detect the silencing effect. The changes in sensitivity to chemotherapeutic drugs of MCF-7/RNAi and its negative control cell lines MCF-7/vector were detected by CCK-8 reagent.
RESULTS: Compared with MCF-7 cell and MCF-7/vector cell, the expression of RABEX-5 mRNA and protein was downregulated in MCF-7/RNAi cells. The sensitivity to epirubicin was reduced after downregulating the expression of RABEX-5, the 50% inhibition concentration (IC50) of MCF-7/RNAi [(3.590 0 +/- 0.228 69) microg/mL] was higher than that of MCF-7/Vvector [(1.193 3 +/- 0.187 71) microg/mL, P < 0.05]; while the same effect was not found in docetaxel group, the IC50 were (11.162 7 +/- 0.210 26) microg/mL and (10.536 7 +/- 0.430 97) microg/mL, respectively (P > 0.05).
CONCLUSION: Downregulation of RABEX-5 can induce chemoresistance to epirubicin in human breast cancer cell MCF-7; while its effect on sensitivity to docetaxel is not significant. This study can provide a theoretical basis for future research RABEX-5 in the individualized treatment of breast cancer.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2013 |
|---|---|
| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:44 |
|---|---|
| Enthalten in: |
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition - 44(2013), 6 vom: 01. Nov., Seite 882-5 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Zhang, Xiang [VerfasserIn] |
|---|
| Themen: |
Anthracyclines |
|---|
| Anmerkungen: |
Date Completed 29.08.2014 Date Revised 04.02.2014 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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|---|
| PPN (Katalog-ID): |
NLM23509658X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
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| 007 | tu | ||
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| 100 | 1 | |a Zhang, Xiang |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The effect of RABEX-5 downregulation on the chemosensitivity of human breast cancer cells |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 29.08.2014 | ||
| 500 | |a Date Revised 04.02.2014 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To investigate the changes in sensitivity to anthracycline and taxanes of human breast cancer cells after RABEX-5 downregulated | ||
| 520 | |a METHODS: By constructing a lentiviral vector for RNA interference (RNAi) of RABEX-5 gene and transfected into human breast cancer cell line MCF-7 to silence the express of RABEX-5. Real-time PCR and Western blot were used to detect the silencing effect. The changes in sensitivity to chemotherapeutic drugs of MCF-7/RNAi and its negative control cell lines MCF-7/vector were detected by CCK-8 reagent | ||
| 520 | |a RESULTS: Compared with MCF-7 cell and MCF-7/vector cell, the expression of RABEX-5 mRNA and protein was downregulated in MCF-7/RNAi cells. The sensitivity to epirubicin was reduced after downregulating the expression of RABEX-5, the 50% inhibition concentration (IC50) of MCF-7/RNAi [(3.590 0 +/- 0.228 69) microg/mL] was higher than that of MCF-7/Vvector [(1.193 3 +/- 0.187 71) microg/mL, P < 0.05]; while the same effect was not found in docetaxel group, the IC50 were (11.162 7 +/- 0.210 26) microg/mL and (10.536 7 +/- 0.430 97) microg/mL, respectively (P > 0.05) | ||
| 520 | |a CONCLUSION: Downregulation of RABEX-5 can induce chemoresistance to epirubicin in human breast cancer cell MCF-7; while its effect on sensitivity to docetaxel is not significant. This study can provide a theoretical basis for future research RABEX-5 in the individualized treatment of breast cancer | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Anthracyclines |2 NLM | |
| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 650 | 7 | |a Guanine Nucleotide Exchange Factors |2 NLM | |
| 650 | 7 | |a RABGEF1 protein, human |2 NLM | |
| 650 | 7 | |a Taxoids |2 NLM | |
| 700 | 1 | |a Li, Hong-Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Min, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Xin-Jie |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition |d 2003 |g 44(2013), 6 vom: 01. Nov., Seite 882-5 |w (DE-627)NLM126647062 |x 1672-173X |7 nnns |
| 773 | 1 | 8 | |g volume:44 |g year:2013 |g number:6 |g day:01 |g month:11 |g pages:882-5 |
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