Pharmacological characterisation of ligand- and voltage-gated ion channels expressed in human iPSC-derived forebrain neurons
INTRODUCTION: Genetic causes, or predisposition, are increasingly accepted to be part of the ethiopathogenesis of many neuropsychiatric diseases. While genes can be studied in any type of cells, their physiological function in human brain cells is difficult to evaluate, particularly in living subjects.
METHODS: As a first step towards the characterisation of human inducible pluripotent stem cell (iPSC)-derived neurons from autism spectrum disorder (ASD) patients, we used gene expression and functional studies to define the regional identity of the typical forebrain differentiation, demonstrate expression patterns of genes of interest in ASD and understand the properties of 'control' iPSC-derived neurons (iCell-Neurons™), with a focus on receptors and ion channels that play a central role in synaptic physio-pathology.
RESULTS AND DISCUSSION: The gene expression profile of the iCell-Neurons™ closely resembled that observed in neonatal prefrontal cortex tissues. Functional studies, performed mainly using calcium flux assays, demonstrated the presence of ionotropic glutamate (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and N-methyl-D-aspartate) and gamma-aminobutyric acid type A receptors. Voltage-gated sodium and calcium channels were also identified using similar techniques.
CONCLUSIONS: Overall, the results reported here suggest that iCell-Neurons™ are a good cellular model of a relatively immature forebrain human neuron population that can be used both as a control in comparison to patients cells, and as host cells in which mutations, insertions and deletions can be used in order to study the molecular mechanisms of ASD and other neurological disorders in an isogenic cellular background.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:231 |
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| Enthalten in: |
Psychopharmacology - 231(2014), 6 vom: 16. März, Seite 1105-24 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dage, Jeffrey L [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.11.2014 Date Revised 09.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00213-013-3384-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM234530561 |
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| 100 | 1 | |a Dage, Jeffrey L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Pharmacological characterisation of ligand- and voltage-gated ion channels expressed in human iPSC-derived forebrain neurons |
| 264 | 1 | |c 2014 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 12.11.2014 | ||
| 500 | |a Date Revised 09.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: Genetic causes, or predisposition, are increasingly accepted to be part of the ethiopathogenesis of many neuropsychiatric diseases. While genes can be studied in any type of cells, their physiological function in human brain cells is difficult to evaluate, particularly in living subjects | ||
| 520 | |a METHODS: As a first step towards the characterisation of human inducible pluripotent stem cell (iPSC)-derived neurons from autism spectrum disorder (ASD) patients, we used gene expression and functional studies to define the regional identity of the typical forebrain differentiation, demonstrate expression patterns of genes of interest in ASD and understand the properties of 'control' iPSC-derived neurons (iCell-Neurons™), with a focus on receptors and ion channels that play a central role in synaptic physio-pathology | ||
| 520 | |a RESULTS AND DISCUSSION: The gene expression profile of the iCell-Neurons™ closely resembled that observed in neonatal prefrontal cortex tissues. Functional studies, performed mainly using calcium flux assays, demonstrated the presence of ionotropic glutamate (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid and N-methyl-D-aspartate) and gamma-aminobutyric acid type A receptors. Voltage-gated sodium and calcium channels were also identified using similar techniques | ||
| 520 | |a CONCLUSIONS: Overall, the results reported here suggest that iCell-Neurons™ are a good cellular model of a relatively immature forebrain human neuron population that can be used both as a control in comparison to patients cells, and as host cells in which mutations, insertions and deletions can be used in order to study the molecular mechanisms of ASD and other neurological disorders in an isogenic cellular background | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Calcium Channels |2 NLM | |
| 650 | 7 | |a Ion Channels |2 NLM | |
| 650 | 7 | |a RNA, Messenger |2 NLM | |
| 650 | 7 | |a Receptors, GABA |2 NLM | |
| 650 | 7 | |a Receptors, GABA-A |2 NLM | |
| 650 | 7 | |a Receptors, Ionotropic Glutamate |2 NLM | |
| 650 | 7 | |a Receptors, N-Methyl-D-Aspartate |2 NLM | |
| 650 | 7 | |a Voltage-Gated Sodium Channels |2 NLM | |
| 650 | 7 | |a Calcium |2 NLM | |
| 650 | 7 | |a SY7Q814VUP |2 NLM | |
| 700 | 1 | |a Colvin, Ellen M |e verfasserin |4 aut | |
| 700 | 1 | |a Fouillet, Antoine |e verfasserin |4 aut | |
| 700 | 1 | |a Langron, Emily |e verfasserin |4 aut | |
| 700 | 1 | |a Roell, William C |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jingling |e verfasserin |4 aut | |
| 700 | 1 | |a Mathur, Sachin X |e verfasserin |4 aut | |
| 700 | 1 | |a Mogg, Adrian J |e verfasserin |4 aut | |
| 700 | 1 | |a Schmitt, Matthew G |e verfasserin |4 aut | |
| 700 | 1 | |a Felder, Christian C |e verfasserin |4 aut | |
| 700 | 1 | |a Merchant, Kalpana M |e verfasserin |4 aut | |
| 700 | 1 | |a Isaac, John |e verfasserin |4 aut | |
| 700 | 1 | |a Broad, Lisa M |e verfasserin |4 aut | |
| 700 | 1 | |a Sher, Emanuele |e verfasserin |4 aut | |
| 700 | 1 | |a Ursu, Daniel |e verfasserin |4 aut | |
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