Phylogenetic analysis of bacterial community in the gut of American cockroach (Periplaneta americana)
OBJECTIVE: The present study was to fully evaluate the intestinal bacterial community of Periplaneta americana, an important model to study insects.
METHODS: We investigated the bacterial community of P. americana gut by culture-independent methods, involving constructing the 16S rRNA gene library and microbial diversity analysis.
RESULTS: The phylotypes were affiliated with Proteobacteria (66.4%), Bacteroidetes (17.8%), Firmicutes (14.5%), Fusobacteria (0.6%) and unclassified bacteria (0.6%). Phylogenetic analysis shows that 15% of the sequences clustered with that from a closely related omnivorous cockroach; and 59% clustered with that from more distantly related animals, including omnivorous, herbivorous, and carnivorous animals, which differ greatly in feeding habits. Moreover, 18% of the clones showed high sequence identity with potential pathogens closely related to human diseases, which also reinforces the concept of the cockroach as a carrier of pathogens.
CONCLUSION: Due to their habits of feeding on a variety of foodstuffs, omnivorous cockroaches harbor a large and diverse microbial community in the gut. The host phylogeny and dietary habits might be critical for the intestinal bacterial community composition of cockroaches.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Wei sheng wu xue bao = Acta microbiologica Sinica - 53(2013), 9 vom: 04. Sept., Seite 984-94 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Fang, Wei [VerfasserIn] |
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| Themen: |
DNA, Bacterial |
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| Anmerkungen: |
Date Completed 23.01.2014 Date Revised 31.12.2013 published: Print GENBANK: JX457153, JX457154, JX457155, JX457156, JX457157, JX457158, JX457159, JX457160, JX457161, JX457162, JX457163, JX457164, JX457165, JX457166, JX457167, JX457168, JX457169, JX457170, JX457171, JX457172, JX457173, JX457174, JX457175, JX457176, JX457177, JX457178, JX457179, JX457180, JX457181, JX457182, JX457183, JX457184, JX457185, JX457186, JX457187, JX457188, JX457189, JX457190, JX457191, JX457192, JX457193, JX457194, JX457195, JX457196, JX457197, JX457198, JX457199, JX457200, JX457201, JX457202, JX457203, JX457204, JX457205, JX457206, JX457207, JX457208, JX457209, JX457210, JX457211, JX457212, JX457213, JX457214, JX457215, JX457216, JX457217, JX457218, JX457219, JX457220, JX457221, JX457222, JX457223, JX457224, JX457225, JX457226, JX457227, JX457228, JX457229, JX457230, JX457231, JX457232, JX457233, JX457234, JX457235, JX457236, JX457237, JX457238, JX457239, JX457240, JX457241, JX457242, JX457243, JX457244, JX457245, JX457246, JX457247, JX457248, JX457249, JX457250, JX457251, JX457252, JX457253, JX457254, JX457255, JX457256, JX457257, JX457258, JX457259, JX457260, JX457261, JX457262, JX457263, JX457264, JX457265, JX457266, JX457267, JX457268, JX457269, JX457270, JX457271, JX457272, JX457273, JX457274, JX457275, JX457276, JX457277, JX457278, JX457279, JX457280, JX457281, JX457282, JX457283, JX457284, JX457285, JX457286, JX457287, JX457288, JX457289, JX457290, JX457291, JX457292, JX457293, JX457294, JX457295, JX457296, JX457297, JX457298, JX457299, JX457300, JX457301, JX457302, JX457303, JX457304, JX457305, JX457306, JX457307, JX457308, JX457309, JX457310, JX457311, JX457312, JX457313, JX457314, JX457315, JX457316, JX457317 Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM234033134 |
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| LEADER | 01000naa a22002652 4500 | ||
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| 008 | 231224s2013 xx ||||| 00| ||eng c | ||
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| 035 | |a (NLM)24377251 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Fang, Wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Phylogenetic analysis of bacterial community in the gut of American cockroach (Periplaneta americana) |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 23.01.2014 | ||
| 500 | |a Date Revised 31.12.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a GENBANK: JX457153, JX457154, JX457155, JX457156, JX457157, JX457158, JX457159, JX457160, JX457161, JX457162, JX457163, JX457164, JX457165, JX457166, JX457167, JX457168, JX457169, JX457170, JX457171, JX457172, JX457173, JX457174, JX457175, JX457176, JX457177, JX457178, JX457179, JX457180, JX457181, JX457182, JX457183, JX457184, JX457185, JX457186, JX457187, JX457188, JX457189, JX457190, JX457191, JX457192, JX457193, JX457194, JX457195, JX457196, JX457197, JX457198, JX457199, JX457200, JX457201, JX457202, JX457203, JX457204, JX457205, JX457206, JX457207, JX457208, JX457209, JX457210, JX457211, JX457212, JX457213, JX457214, JX457215, JX457216, JX457217, JX457218, JX457219, JX457220, JX457221, JX457222, JX457223, JX457224, JX457225, JX457226, JX457227, JX457228, JX457229, JX457230, JX457231, JX457232, JX457233, JX457234, JX457235, JX457236, JX457237, JX457238, JX457239, JX457240, JX457241, JX457242, JX457243, JX457244, JX457245, JX457246, JX457247, JX457248, JX457249, JX457250, JX457251, JX457252, JX457253, JX457254, JX457255, JX457256, JX457257, JX457258, JX457259, JX457260, JX457261, JX457262, JX457263, JX457264, JX457265, JX457266, JX457267, JX457268, JX457269, JX457270, JX457271, JX457272, JX457273, JX457274, JX457275, JX457276, JX457277, JX457278, JX457279, JX457280, JX457281, JX457282, JX457283, JX457284, JX457285, JX457286, JX457287, JX457288, JX457289, JX457290, JX457291, JX457292, JX457293, JX457294, JX457295, JX457296, JX457297, JX457298, JX457299, JX457300, JX457301, JX457302, JX457303, JX457304, JX457305, JX457306, JX457307, JX457308, JX457309, JX457310, JX457311, JX457312, JX457313, JX457314, JX457315, JX457316, JX457317 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: The present study was to fully evaluate the intestinal bacterial community of Periplaneta americana, an important model to study insects | ||
| 520 | |a METHODS: We investigated the bacterial community of P. americana gut by culture-independent methods, involving constructing the 16S rRNA gene library and microbial diversity analysis | ||
| 520 | |a RESULTS: The phylotypes were affiliated with Proteobacteria (66.4%), Bacteroidetes (17.8%), Firmicutes (14.5%), Fusobacteria (0.6%) and unclassified bacteria (0.6%). Phylogenetic analysis shows that 15% of the sequences clustered with that from a closely related omnivorous cockroach; and 59% clustered with that from more distantly related animals, including omnivorous, herbivorous, and carnivorous animals, which differ greatly in feeding habits. Moreover, 18% of the clones showed high sequence identity with potential pathogens closely related to human diseases, which also reinforces the concept of the cockroach as a carrier of pathogens | ||
| 520 | |a CONCLUSION: Due to their habits of feeding on a variety of foodstuffs, omnivorous cockroaches harbor a large and diverse microbial community in the gut. The host phylogeny and dietary habits might be critical for the intestinal bacterial community composition of cockroaches | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a DNA, Bacterial |2 NLM | |
| 650 | 7 | |a DNA, Ribosomal |2 NLM | |
| 650 | 7 | |a RNA, Ribosomal, 16S |2 NLM | |
| 700 | 1 | |a Fang, Zemin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Zhimiao |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xuecheng |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Yuzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Yazhong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Wei sheng wu xue bao = Acta microbiologica Sinica |d 1985 |g 53(2013), 9 vom: 04. Sept., Seite 984-94 |w (DE-627)NLM012631655 |x 0001-6209 |7 nnns |
| 773 | 1 | 8 | |g volume:53 |g year:2013 |g number:9 |g day:04 |g month:09 |g pages:984-94 |
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