Effect of trace tetracycline concentrations on the structure of a microbial community and the development of tetracycline resistance genes in sequencing batch reactors
Copyright © 2013 Elsevier Ltd. All rights reserved..
The objective of this study was to investigate effects of different concentrations of tetracycline (TC) on the microbial community and development of tetracycline resistance genes (TRGs) of sequencing batch reactors (SBRs). Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis of 16S rRNA and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) were used to detect the structural changes of the microbial community and the variations of eight TC resistance genes tet(A), tet(B), tet(C), tet(E), tet(M), tet(O), tet(S) and tet(X), respectively. The results indicated that, trace TC could substantially change the structure of the microbial community. Bacteria which could not adapt to environment with TC were gradually replaced by those adapting to tetracycline. Shannon's diversity index (H) and Simpson's index (D) reached maximum values when the concentration of TC was 1 μg L(-1). The resistance genes in the activated sludge proliferated under the pressure of trace TC.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:150 |
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| Enthalten in: |
Bioresource technology - 150(2013) vom: 01. Dez., Seite 9-14 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Wei [VerfasserIn] |
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| Links: |
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| Themen: |
Activated sludge |
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| Anmerkungen: |
Date Completed 07.08.2014 Date Revised 02.12.2013 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2013.09.081 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM231843275 |
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| 245 | 1 | 0 | |a Effect of trace tetracycline concentrations on the structure of a microbial community and the development of tetracycline resistance genes in sequencing batch reactors |
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| 500 | |a Date Revised 02.12.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2013 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The objective of this study was to investigate effects of different concentrations of tetracycline (TC) on the microbial community and development of tetracycline resistance genes (TRGs) of sequencing batch reactors (SBRs). Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) analysis of 16S rRNA and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) were used to detect the structural changes of the microbial community and the variations of eight TC resistance genes tet(A), tet(B), tet(C), tet(E), tet(M), tet(O), tet(S) and tet(X), respectively. The results indicated that, trace TC could substantially change the structure of the microbial community. Bacteria which could not adapt to environment with TC were gradually replaced by those adapting to tetracycline. Shannon's diversity index (H) and Simpson's index (D) reached maximum values when the concentration of TC was 1 μg L(-1). The resistance genes in the activated sludge proliferated under the pressure of trace TC | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Activated sludge | |
| 650 | 4 | |a Microbial community | |
| 650 | 4 | |a Resistance genes | |
| 650 | 4 | |a SBR | |
| 650 | 4 | |a Tetracycline | |
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| 700 | 1 | |a Qi, Fang-fang |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Pei-zhe |e verfasserin |4 aut | |
| 700 | 1 | |a Van Ginkel, Steven W |e verfasserin |4 aut | |
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