Isolation of RNA from equine peripheral blood cells : comparison of methods
Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, LeukoLOCK™ total RNA isolation system was the best RNA extraction method.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
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| Enthalten in: |
SpringerPlus - 2(2013) vom: 26., Seite 478 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jiang, Zibin [VerfasserIn] |
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| Links: |
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| Themen: |
Blood |
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| Anmerkungen: |
Date Completed 17.10.2013 Date Revised 21.10.2021 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1186/2193-1801-2-478 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Gene expression studies in equine research involve the use of whole blood samples as a vital source of RNA. To determine the optimal method for RNA isolation from equine whole blood, we compared three RNA isolation strategies using different commercially available kits to evaluate the yield and quality of equine RNA. All 3 methods produced RNA with high quality. Though it did not produce the highest yield, combining the quality, yield and the need for the downstream application in our project, LeukoLOCK™ total RNA isolation system was the best RNA extraction method | ||
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