Nuclear structures surrounding internal lamin invaginations
© 2013 Wiley Periodicals, Inc..
A- and C-type lamins are intermediate filament proteins responsible for the maintenance of nuclear shape and most likely nuclear architecture. Here, we propose that pronounced invaginations of A/C-type lamins into the nuclear interior represent channels for the transport of regulatory molecules to and from nuclear and nucleolar regions. Using fluorescent protein technology and immunofluorescence, we show that A-type lamin channels interact with several nuclear components, including fibrillarin- and UBF-positive regions of nucleoli, foci of heterochromatin protein 1 β, polycomb group bodies, and genomic regions associated with DNA repair. Similar associations were observed between A/C-type lamin channels and nuclear pores, lamin-associated protein LAP2α, and promyelocytic leukemia nuclear bodies. Interestingly, regions with high levels of A/C-type lamins had low levels of B-type lamins, and vice versa. These characteristics were observed in primary and immortalized mouse embryonic fibroblasts as well as human and mouse embryonic stem cell colonies exhibiting stem cell-specific lamin positivity. Our findings indicate that internal channels formed by nuclear lamins likely contribute to normal cellular processes through association with various nuclear and nucleolar structures.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2014 |
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| Erschienen: |
2014 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:115 |
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| Enthalten in: |
Journal of cellular biochemistry - 115(2014), 3 vom: 23. März, Seite 476-87 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Legartová, Soňa [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 20.10.2014 Date Revised 04.03.2014 published: Print Citation Status MEDLINE |
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| doi: |
10.1002/jcb.24681 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2013 Wiley Periodicals, Inc. | ||
| 520 | |a A- and C-type lamins are intermediate filament proteins responsible for the maintenance of nuclear shape and most likely nuclear architecture. Here, we propose that pronounced invaginations of A/C-type lamins into the nuclear interior represent channels for the transport of regulatory molecules to and from nuclear and nucleolar regions. Using fluorescent protein technology and immunofluorescence, we show that A-type lamin channels interact with several nuclear components, including fibrillarin- and UBF-positive regions of nucleoli, foci of heterochromatin protein 1 β, polycomb group bodies, and genomic regions associated with DNA repair. Similar associations were observed between A/C-type lamin channels and nuclear pores, lamin-associated protein LAP2α, and promyelocytic leukemia nuclear bodies. Interestingly, regions with high levels of A/C-type lamins had low levels of B-type lamins, and vice versa. These characteristics were observed in primary and immortalized mouse embryonic fibroblasts as well as human and mouse embryonic stem cell colonies exhibiting stem cell-specific lamin positivity. Our findings indicate that internal channels formed by nuclear lamins likely contribute to normal cellular processes through association with various nuclear and nucleolar structures | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CHROMATIN | |
| 650 | 4 | |a DNA REPAIR | |
| 650 | 4 | |a ES CELLS | |
| 650 | 4 | |a HP1 PROTEIN | |
| 650 | 4 | |a LAMINS | |
| 650 | 4 | |a NUCLEAR PORES | |
| 650 | 4 | |a PML BODIES | |
| 650 | 4 | |a TRANSCRIPTION | |
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| 650 | 7 | |a Lamin Type A |2 NLM | |
| 650 | 7 | |a Lamin Type B |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a fibrillarin |2 NLM | |
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| 700 | 1 | |a Stixová, Lenka |e verfasserin |4 aut | |
| 700 | 1 | |a Laur, Oskar |e verfasserin |4 aut | |
| 700 | 1 | |a Kozubek, Stanislav |e verfasserin |4 aut | |
| 700 | 1 | |a Sehnalová, Petra |e verfasserin |4 aut | |
| 700 | 1 | |a Bártová, Eva |e verfasserin |4 aut | |
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