A HCN4+ cardiomyogenic progenitor derived from the first heart field and human pluripotent stem cells
Most of the mammalian heart is formed from mesodermal progenitors in the first and second heart fields (FHF and SHF), whereby the FHF gives rise to the left ventricle and parts of the atria and the SHF to the right ventricle, outflow tract and parts of the atria. Whereas SHF progenitors have been characterized in detail, using specific molecular markers, comprehensive studies on the FHF have been hampered by the lack of exclusive markers. Here, we present Hcn4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) as an FHF marker. Lineage-traced Hcn4+/FHF cells delineate FHF-derived structures in the heart and primarily contribute to cardiomyogenic cell lineages, thereby identifying an early cardiomyogenic progenitor pool. As a surface marker, HCN4 also allowed the isolation of cardiomyogenic Hcn4+/FHF progenitors from human embryonic stem cells. We conclude that a primary purpose of the FHF is to generate cardiac muscle and support the contractile activity of the primitive heart tube, whereas SHF-derived progenitors contribute to heart cell lineage diversification.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2013 |
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Erschienen: |
2013 |
Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
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Enthalten in: |
Nature cell biology - 15(2013), 9 vom: 20. Sept., Seite 1098-106 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Später, Daniela [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 13.11.2013 Date Revised 21.10.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1038/ncb2824 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM230299067 |
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520 | |a Most of the mammalian heart is formed from mesodermal progenitors in the first and second heart fields (FHF and SHF), whereby the FHF gives rise to the left ventricle and parts of the atria and the SHF to the right ventricle, outflow tract and parts of the atria. Whereas SHF progenitors have been characterized in detail, using specific molecular markers, comprehensive studies on the FHF have been hampered by the lack of exclusive markers. Here, we present Hcn4 (hyperpolarization-activated cyclic nucleotide-gated channel 4) as an FHF marker. Lineage-traced Hcn4+/FHF cells delineate FHF-derived structures in the heart and primarily contribute to cardiomyogenic cell lineages, thereby identifying an early cardiomyogenic progenitor pool. As a surface marker, HCN4 also allowed the isolation of cardiomyogenic Hcn4+/FHF progenitors from human embryonic stem cells. We conclude that a primary purpose of the FHF is to generate cardiac muscle and support the contractile activity of the primitive heart tube, whereas SHF-derived progenitors contribute to heart cell lineage diversification | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, N.I.H., Extramural | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 7 | |a Biomarkers |2 NLM | |
650 | 7 | |a Cyclic Nucleotide-Gated Cation Channels |2 NLM | |
650 | 7 | |a HCN4 protein, human |2 NLM | |
650 | 7 | |a Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels |2 NLM | |
650 | 7 | |a Muscle Proteins |2 NLM | |
650 | 7 | |a Potassium Channels |2 NLM | |
700 | 1 | |a Abramczuk, Monika K |e verfasserin |4 aut | |
700 | 1 | |a Buac, Kristina |e verfasserin |4 aut | |
700 | 1 | |a Zangi, Lior |e verfasserin |4 aut | |
700 | 1 | |a Stachel, Maxine W |e verfasserin |4 aut | |
700 | 1 | |a Clarke, Jonathan |e verfasserin |4 aut | |
700 | 1 | |a Sahara, Makoto |e verfasserin |4 aut | |
700 | 1 | |a Ludwig, Andreas |e verfasserin |4 aut | |
700 | 1 | |a Chien, Kenneth R |e verfasserin |4 aut | |
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