Ambulatory gastric lavages provide better yields of Mycobacterium tuberculosis than induced sputum in children with intrathoracic tuberculosis
OBJECTIVE: To compare mycobacterial yield by induced sputum (IS) and gastric lavage (GL), performed on an ambulatory basis in children with probable intrathoracic tuberculosis.
DESIGN: Diagnostic accuracy study.
SETTING: Two tertiary care hospitals in Delhi, India.
PATIENTS: Children aged 6 months to 15 years with newly diagnosed intrathoracic tuberculosis.
METHODS: GL and IS were performed in children on 2 consecutive days on ambulatory basis. Samples were examined by Ziehl-Neelsen staining and cultured on an automated BACTEC-MGIT 960 system.
OUTCOME MEASURE: Mycobacterial yields (smear and culture) for the 2 sample types (IS and GL) were compared.
RESULTS: Four hundred three children (56.6% girls), median age 111 months (interquartile range: 68, 144) were enrolled. Overall yield for acid-fast bacilli and/or Mycobacterium tuberculosis (MTB) by either IS and/or GL was 152 (37.7%). Acid-fast bacilli positivity from IS and GL were 5.7% (23) and 10.4% (42), respectively. Confirmed MTB on culture from IS and GL were 17.9% (72) and 32.5% (127), respectively (P < 0.001). IS and GL identified 17 (4.2%) and 73 (18.1%) additional cases respectively when the other method failed to identify MTB. The combined yields (acid-fast bacilli positivity/MTB) with GL and IS on day 1 (115, 28.5%) were less than that obtained from 2 consecutive GL (135, 33.5%), but better than 2 consecutive IS samples (79, 19.6%; P < 0.001).
CONCLUSION: It is feasible to collect induced sputum and gastric lavage on an ambulatory basis. The yield of MTB obtained by GL is superior to that obtained by IS.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2013 |
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Erschienen: |
2013 |
Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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Enthalten in: |
The Pediatric infectious disease journal - 32(2013), 12 vom: 07. Dez., Seite 1313-7 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Mukherjee, Aparna [VerfasserIn] |
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Links: |
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Themen: |
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Anmerkungen: |
Date Completed 30.03.2015 Date Revised 27.02.2014 published: Print Citation Status MEDLINE |
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doi: |
10.1097/INF.0b013e31829f5c58 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM230154077 |
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100 | 1 | |a Mukherjee, Aparna |e verfasserin |4 aut | |
245 | 1 | 0 | |a Ambulatory gastric lavages provide better yields of Mycobacterium tuberculosis than induced sputum in children with intrathoracic tuberculosis |
264 | 1 | |c 2013 | |
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500 | |a Date Completed 30.03.2015 | ||
500 | |a Date Revised 27.02.2014 | ||
500 | |a published: Print | ||
500 | |a Citation Status MEDLINE | ||
520 | |a OBJECTIVE: To compare mycobacterial yield by induced sputum (IS) and gastric lavage (GL), performed on an ambulatory basis in children with probable intrathoracic tuberculosis | ||
520 | |a DESIGN: Diagnostic accuracy study | ||
520 | |a SETTING: Two tertiary care hospitals in Delhi, India | ||
520 | |a PATIENTS: Children aged 6 months to 15 years with newly diagnosed intrathoracic tuberculosis | ||
520 | |a METHODS: GL and IS were performed in children on 2 consecutive days on ambulatory basis. Samples were examined by Ziehl-Neelsen staining and cultured on an automated BACTEC-MGIT 960 system | ||
520 | |a OUTCOME MEASURE: Mycobacterial yields (smear and culture) for the 2 sample types (IS and GL) were compared | ||
520 | |a RESULTS: Four hundred three children (56.6% girls), median age 111 months (interquartile range: 68, 144) were enrolled. Overall yield for acid-fast bacilli and/or Mycobacterium tuberculosis (MTB) by either IS and/or GL was 152 (37.7%). Acid-fast bacilli positivity from IS and GL were 5.7% (23) and 10.4% (42), respectively. Confirmed MTB on culture from IS and GL were 17.9% (72) and 32.5% (127), respectively (P < 0.001). IS and GL identified 17 (4.2%) and 73 (18.1%) additional cases respectively when the other method failed to identify MTB. The combined yields (acid-fast bacilli positivity/MTB) with GL and IS on day 1 (115, 28.5%) were less than that obtained from 2 consecutive GL (135, 33.5%), but better than 2 consecutive IS samples (79, 19.6%; P < 0.001) | ||
520 | |a CONCLUSION: It is feasible to collect induced sputum and gastric lavage on an ambulatory basis. The yield of MTB obtained by GL is superior to that obtained by IS | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
700 | 1 | |a Singh, Sarman |e verfasserin |4 aut | |
700 | 1 | |a Lodha, Rakesh |e verfasserin |4 aut | |
700 | 1 | |a Singh, Varinder |e verfasserin |4 aut | |
700 | 1 | |a Hesseling, A C |e verfasserin |4 aut | |
700 | 1 | |a Grewal, Harleen M S |e verfasserin |4 aut | |
700 | 1 | |a Kabra, Sushil K |e verfasserin |4 aut | |
700 | 0 | |a Delhi Pediatric TB Study Group |e verfasserin |4 aut | |
700 | 1 | |a Aneja, S |e investigator |4 oth | |
700 | 1 | |a Arya, Tina |e investigator |4 oth | |
700 | 1 | |a Bhatnagar, S |e investigator |4 oth | |
700 | 1 | |a Chandra, J |e investigator |4 oth | |
700 | 1 | |a Dutta, A K |e investigator |4 oth | |
700 | 1 | |a Doherty, T M |e investigator |4 oth | |
700 | 1 | |a Friis, H |e investigator |4 oth | |
700 | 1 | |a Grewal, Harleen M S |e investigator |4 oth | |
700 | 1 | |a Hesseling, A C |e investigator |4 oth | |
700 | 1 | |a Kabra, S K |e investigator |4 oth | |
700 | 1 | |a Lodha, Rakesh |e investigator |4 oth | |
700 | 1 | |a Marais, B |e investigator |4 oth | |
700 | 1 | |a Mukherjee, Aparna |e investigator |4 oth | |
700 | 1 | |a Parashar, Deepak |e investigator |4 oth | |
700 | 1 | |a Prajapati, Suneel |e investigator |4 oth | |
700 | 1 | |a Purohit, Kamna |e investigator |4 oth | |
700 | 1 | |a Saini, Deepak |e investigator |4 oth | |
700 | 1 | |a Saini, Savita |e investigator |4 oth | |
700 | 1 | |a Singh, Ravi Raj |e investigator |4 oth | |
700 | 1 | |a Singh, Sarman |e investigator |4 oth | |
700 | 1 | |a Singh, Varinder |e investigator |4 oth | |
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