Heparin-binding haemagglutinin, a new tool for the detection of latent Mycobacterium tuberculosis infection in hemodialysis patients
BACKGROUND: Patients with end-stage renal disease (ESRD) and latently infected with Mycobacterium tuberculosis (LTBI) are at higher risk to develop tuberculosis (TB) than healthy subjects. Interferon-gamma release assays (IGRAs) were reported to be more sensitive than tuberculin skin tests for the detection of infected individuals in dialysis patients.
METHODS: On 143 dialysis patients prospectively enrolled, we compared the results from the QuantiFERON®-TB Gold assay (QFT), to those of an IGRA in response to in vitro stimulation of circulating mononuclear cells with the mycobacterial latency antigen Heparin-Binding Haemagglutinin purified from Mycobacterium bovis BCG (native HBHA, nHBHA).
RESULTS: Seven patients had a past history of active TB and 1 had an undetermined result with both IGRAs. Among the other 135 patients, 94 had concordant results with the QFT and nHBHA-IGRA, 40.0% being negative and therefore not latently infected, and 29.6% being positive and thus LTBI. Discrepant results between these tests were found for 36 patients positive only with the nHBHA-IGRA and 5 only with the QFT.
CONCLUSIONS: The nHBHA-IGRA is more sensitive than the QFT for the detection of LTBI dialysis patients, and follow-up of the patients will allow us to define the clinical significance of discrepant results between the nHBHA-IGRA and the QFT.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
PloS one - 8(2013), 8 vom: 01., Seite e71088 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dessein, Rodrigue [VerfasserIn] |
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| Links: |
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| Themen: |
Antigens, Bacterial |
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| Anmerkungen: |
Date Completed 03.03.2014 Date Revised 21.10.2021 published: Electronic-Print Citation Status MEDLINE |
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| doi: |
10.1371/journal.pone.0071088 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM229984207 |
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| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Electronic-Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a BACKGROUND: Patients with end-stage renal disease (ESRD) and latently infected with Mycobacterium tuberculosis (LTBI) are at higher risk to develop tuberculosis (TB) than healthy subjects. Interferon-gamma release assays (IGRAs) were reported to be more sensitive than tuberculin skin tests for the detection of infected individuals in dialysis patients | ||
| 520 | |a METHODS: On 143 dialysis patients prospectively enrolled, we compared the results from the QuantiFERON®-TB Gold assay (QFT), to those of an IGRA in response to in vitro stimulation of circulating mononuclear cells with the mycobacterial latency antigen Heparin-Binding Haemagglutinin purified from Mycobacterium bovis BCG (native HBHA, nHBHA) | ||
| 520 | |a RESULTS: Seven patients had a past history of active TB and 1 had an undetermined result with both IGRAs. Among the other 135 patients, 94 had concordant results with the QFT and nHBHA-IGRA, 40.0% being negative and therefore not latently infected, and 29.6% being positive and thus LTBI. Discrepant results between these tests were found for 36 patients positive only with the nHBHA-IGRA and 5 only with the QFT | ||
| 520 | |a CONCLUSIONS: The nHBHA-IGRA is more sensitive than the QFT for the detection of LTBI dialysis patients, and follow-up of the patients will allow us to define the clinical significance of discrepant results between the nHBHA-IGRA and the QFT | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, Bacterial |2 NLM | |
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| 650 | 7 | |a heparin-binding hemagglutinin |2 NLM | |
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| 700 | 1 | |a Nortier, Joëlle |e verfasserin |4 aut | |
| 700 | 1 | |a Dratwa, Max |e verfasserin |4 aut | |
| 700 | 1 | |a Gastaldello, Karine |e verfasserin |4 aut | |
| 700 | 1 | |a Pozdzik, Agnieszka |e verfasserin |4 aut | |
| 700 | 1 | |a Lecher, Sophie |e verfasserin |4 aut | |
| 700 | 1 | |a Grandbastien, Bruno |e verfasserin |4 aut | |
| 700 | 1 | |a Locht, Camille |e verfasserin |4 aut | |
| 700 | 1 | |a Mascart, Françoise |e verfasserin |4 aut | |
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