Evaluation of the SD Dengue Duo diagnosis system for detection of NS1 protein and IgM and IgG dengue antibodies
INTRODUCTION: SD Dengue Duo (Standard Diagnosis) commercial kit is an immunochromatographic rapid test that detects NS1 protein and IgG/IgM dengue antibodies simultaneously.
OBJECTIVE: to evaluate the operational and functional characteristics of this system for the detection of virological and serological markers.
METHODS: sera panel was made up by 161 samples, 113 from patients with clinically and serologically confirmed dengue caused by any of the four dengue virus serotypes and 48 negative samples. All these samples were tested by SD Dengue Duo Kit and by Platelia Dengue NSI Ag, IgM Capture ELISA and ELISA Inhibition Method used as reference assays.
RESULTS: the evaluated kit showed a 57.75% sensitivity for the detection of NS1 protein, false negatives were detected in samples collected 5 days or more after fever onset in secondary infection cases. IgM detection showed 96.0% sensitivity and 98.4% specificity. Furthermore, high agreement (95.7%) in classifying dengue infection types (primary or secondary infections) was observed. The global study of the 3 markers, the sensitivity rose to 100%.
CONCLUSIONS: SD Dengue Duo is a simple, easy and rapid assay; it does not require additional equipment, can be used for acute and convalescence serum samples and offers a good alternative for dengue diagnosis in those laboratories where a complete dengue virus diagnosis is difficult to perform.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:64 |
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| Enthalten in: |
Revista cubana de medicina tropical - 64(2012), 1 vom: 01. Jan., Seite 27-34 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Evaluación del sistema diagnóstico SD Dengue Duo para la detección de la proteína NS1 y los anticuerpos IgM e IgG anti-dengue |
|---|
| Beteiligte Personen: |
Valdez Sandoval, José Johanatan [VerfasserIn] |
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| Themen: |
Antibodies, Viral |
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| Anmerkungen: |
Date Completed 01.04.2013 Date Revised 28.02.2013 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM225362074 |
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| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
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| 100 | 1 | |a Valdez Sandoval, José Johanatan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Evaluation of the SD Dengue Duo diagnosis system for detection of NS1 protein and IgM and IgG dengue antibodies |
| 246 | 3 | 3 | |a Evaluación del sistema diagnóstico SD Dengue Duo para la detección de la proteína NS1 y los anticuerpos IgM e IgG anti-dengue |
| 264 | 1 | |c 2012 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 01.04.2013 | ||
| 500 | |a Date Revised 28.02.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: SD Dengue Duo (Standard Diagnosis) commercial kit is an immunochromatographic rapid test that detects NS1 protein and IgG/IgM dengue antibodies simultaneously | ||
| 520 | |a OBJECTIVE: to evaluate the operational and functional characteristics of this system for the detection of virological and serological markers | ||
| 520 | |a METHODS: sera panel was made up by 161 samples, 113 from patients with clinically and serologically confirmed dengue caused by any of the four dengue virus serotypes and 48 negative samples. All these samples were tested by SD Dengue Duo Kit and by Platelia Dengue NSI Ag, IgM Capture ELISA and ELISA Inhibition Method used as reference assays | ||
| 520 | |a RESULTS: the evaluated kit showed a 57.75% sensitivity for the detection of NS1 protein, false negatives were detected in samples collected 5 days or more after fever onset in secondary infection cases. IgM detection showed 96.0% sensitivity and 98.4% specificity. Furthermore, high agreement (95.7%) in classifying dengue infection types (primary or secondary infections) was observed. The global study of the 3 markers, the sensitivity rose to 100% | ||
| 520 | |a CONCLUSIONS: SD Dengue Duo is a simple, easy and rapid assay; it does not require additional equipment, can be used for acute and convalescence serum samples and offers a good alternative for dengue diagnosis in those laboratories where a complete dengue virus diagnosis is difficult to perform | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Immunoglobulin G |2 NLM | |
| 650 | 7 | |a Immunoglobulin M |2 NLM | |
| 650 | 7 | |a Viral Nonstructural Proteins |2 NLM | |
| 700 | 1 | |a Ruiz Amores, Didye |e verfasserin |4 aut | |
| 700 | 1 | |a Vázquez Ramudo, C Susana |e verfasserin |4 aut | |
| 700 | 1 | |a Calzada Gutiérrez, Naifi |e verfasserin |4 aut | |
| 700 | 1 | |a Guzmán Tirado, C Maria G |e verfasserin |4 aut | |
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