Multilocus sequence typing and FlaA sequencing reveal the genetic stability of Campylobacter jejuni enrichment during coculture with Acanthamoeba polyphaga
Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:79 |
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| Enthalten in: |
Applied and environmental microbiology - 79(2013), 7 vom: 01. Apr., Seite 2477-9 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Griekspoor, Petra [VerfasserIn] |
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| Links: |
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| Themen: |
12777-81-0 |
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| Anmerkungen: |
Date Completed 28.08.2013 Date Revised 21.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/AEM.02918-12 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM224723901 |
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| 520 | |a Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity | ||
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