Robotic real-time near infrared targeted fluorescence imaging in a murine model of prostate cancer : a feasibility study
Copyright © 2013 Elsevier Inc. All rights reserved..
OBJECTIVE: To evaluate the detection of near-infrared fluorescence from prostate tumors stained with a prostate-specific membrane antigen (PSMA)-targeted tracer developed in our institution with a novel robotic imaging system.
METHODS: Prostate cancer cell lines PC3-pip (PSMA positive) and PC3-flu (PSMA negative) were implanted subcutaneously into 6 immunodeficient mice. When tumors reached 5 mm, a PSMA-targeted fluorescent conjugate was injected intravenously. The first 3 mice underwent near-infrared imaging immediately and hourly up to 4 hours after injection to determine the time necessary to obtain peak fluorescence and were killed. The last 3 mice were imaged once preoperatively and were euthanized 120 minutes later. Excision of the tumors was performed by using a novel robotic imaging system to detect near-infrared fluorescence in real time. Specimens were submitted for pathology.
RESULTS: In the first 3 mice, we found 120 minutes as the time needed to observe peak fluorescence from the PSMA-positive tumors. We identified discrete near-infrared fluorescence from 2 of 3 PSMA-positive tumors with the robotic imaging system. Surgical margins were negative for all excised specimens except for one PSMA-negative tumor.
CONCLUSIONS: Real-time near-infrared fluorescence imaging of prostate cancer is feasible with a novel robotic imaging system. Further research is needed to optimize the signal intensity detectable from prostate cancer with our tracer. Toxicologic studies are needed before its clinical use.
| Errataetall: |
CommentIn: Urology. 2013 Feb;81(2):456; discussion 457. - PMID 23374829 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:81 |
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| Enthalten in: |
Urology - 81(2013), 2 vom: 02. Feb., Seite 451-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Laydner, Humberto [VerfasserIn] |
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| Links: |
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| Themen: |
EC 3.4.17.21 |
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| Anmerkungen: |
Date Completed 19.04.2013 Date Revised 21.10.2021 published: Print CommentIn: Urology. 2013 Feb;81(2):456; discussion 457. - PMID 23374829 Citation Status MEDLINE |
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| doi: |
10.1016/j.urology.2012.02.075 |
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| PPN (Katalog-ID): |
NLM224693433 |
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| 100 | 1 | |a Laydner, Humberto |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Robotic real-time near infrared targeted fluorescence imaging in a murine model of prostate cancer |b a feasibility study |
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| 500 | |a Date Completed 19.04.2013 | ||
| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a CommentIn: Urology. 2013 Feb;81(2):456; discussion 457. - PMID 23374829 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2013 Elsevier Inc. All rights reserved. | ||
| 520 | |a OBJECTIVE: To evaluate the detection of near-infrared fluorescence from prostate tumors stained with a prostate-specific membrane antigen (PSMA)-targeted tracer developed in our institution with a novel robotic imaging system | ||
| 520 | |a METHODS: Prostate cancer cell lines PC3-pip (PSMA positive) and PC3-flu (PSMA negative) were implanted subcutaneously into 6 immunodeficient mice. When tumors reached 5 mm, a PSMA-targeted fluorescent conjugate was injected intravenously. The first 3 mice underwent near-infrared imaging immediately and hourly up to 4 hours after injection to determine the time necessary to obtain peak fluorescence and were killed. The last 3 mice were imaged once preoperatively and were euthanized 120 minutes later. Excision of the tumors was performed by using a novel robotic imaging system to detect near-infrared fluorescence in real time. Specimens were submitted for pathology | ||
| 520 | |a RESULTS: In the first 3 mice, we found 120 minutes as the time needed to observe peak fluorescence from the PSMA-positive tumors. We identified discrete near-infrared fluorescence from 2 of 3 PSMA-positive tumors with the robotic imaging system. Surgical margins were negative for all excised specimens except for one PSMA-negative tumor | ||
| 520 | |a CONCLUSIONS: Real-time near-infrared fluorescence imaging of prostate cancer is feasible with a novel robotic imaging system. Further research is needed to optimize the signal intensity detectable from prostate cancer with our tracer. Toxicologic studies are needed before its clinical use | ||
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| 700 | 1 | |a Heston, Warren D |e verfasserin |4 aut | |
| 700 | 1 | |a Autorino, Riccardo |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xinning |e verfasserin |4 aut | |
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| 700 | 1 | |a Magi-Galluzzi, Cristina |e verfasserin |4 aut | |
| 700 | 1 | |a Isac, Wahib |e verfasserin |4 aut | |
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| 700 | 1 | |a Hu, Bo |e verfasserin |4 aut | |
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| 700 | 1 | |a Chalikonda, Sricharan |e verfasserin |4 aut | |
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| 700 | 1 | |a Haber, Georges-Pascal |e verfasserin |4 aut | |
| 700 | 1 | |a Kaouk, Jihad H |e verfasserin |4 aut | |
| 700 | 1 | |a Stein, Robert J |e verfasserin |4 aut | |
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