Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system
Copyright © 2012 Elsevier Ltd. All rights reserved..
We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:23 |
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| Enthalten in: |
Bioorganic & medicinal chemistry letters - 23(2013), 4 vom: 15. Feb., Seite 1096-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yoon, Jung-Min [VerfasserIn] |
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| Links: |
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| Themen: |
Gibberellins |
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| Anmerkungen: |
Date Completed 31.10.2013 Date Revised 28.01.2013 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bmcl.2012.12.007 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Copyright © 2012 Elsevier Ltd. All rights reserved. | ||
| 520 | |a We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Gibberellins |2 NLM | |
| 650 | 7 | |a Plant Growth Regulators |2 NLM | |
| 650 | 7 | |a Receptors, Cell Surface |2 NLM | |
| 700 | 1 | |a Nakajima, Masatoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Mashiguchi, Kiyoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Seung-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Otani, Masato |e verfasserin |4 aut | |
| 700 | 1 | |a Asami, Tadao |e verfasserin |4 aut | |
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