Population distribution of Beta-lactamase conferring resistance to third-generation cephalosporins in human clinical Enterobacteriaceae in the Netherlands
There is a global increase in infections caused by Enterobacteriaceae with plasmid-borne β-lactamases that confer resistance to third-generation cephalosporins. The epidemiology of these bacteria is not well understood, and was, therefore, investigated in a selection of 636 clinical Enterobacteriaceae with a minimal inhibitory concentration >1 mg/L for ceftazidime/ceftriaxone from a national survey (75% E. coli, 11% E. cloacae, 11% K. pneumoniae, 2% K. oxytoca, 2% P. mirabilis). Isolates were investigated for extended-spectrum β-lactamases (ESBLs) and ampC genes using microarray, PCR, gene sequencing and molecular straintyping (Diversilab and multi-locus sequence typing (MLST)). ESBL genes were demonstrated in 512 isolates (81%); of which 446 (87%) belonged to the CTX-M family. Among 314 randomly selected and sequenced isolates, bla(CTX-M-15) was most prevalent (n = 124, 39%), followed by bla(CTX-M-1) (n = 47, 15%), bla(CTX-M-14) (n = 15, 5%), bla(SHV-12) (n = 24, 8%) and bla(TEM-52) (n = 13, 4%). Among 181 isolates with MIC ≥16 mg/L for cefoxitin plasmid encoded AmpCs were detected in 32 and 27 were of the CMY-2 group. Among 102 E. coli isolates with MIC ≥16 mg/L for cefoxitin ampC promoter mutations were identified in 29 (28%). Based on Diversilab genotyping of 608 isolates (similarity cut-off >98%) discriminatory indices of bacteria with ESBL and/or ampC genes were 0.994, 0.985 and 0.994 for E. coli, K. pneumoniae and E. cloacae, respectively. Based on similarity cut-off >95% two large clusters of E. coli were apparent (of 43 and 30 isolates) and 21 of 21 that were typed by belonged to ST131 of which 13 contained bla(CTX-M-15). Our findings demonstrate that bla(CTX-M-15) is the most prevalent ESBL and we report a larger than previously reported prevalence of ampC genes among Enterobacteriaceae responsible for resistance to third-generation cephalosporins.
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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| Enthalten in: |
PloS one - 7(2012), 12 vom: 01., Seite e52102 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Voets, Guido M [VerfasserIn] |
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| Links: |
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| Themen: |
AmpC beta-lactamases |
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| Anmerkungen: |
Date Completed 25.06.2013 Date Revised 21.10.2021 published: Print-Electronic ErratumIn: PLoS One. 2015;9(9):107786 Hall, Maurine A L [corrected to Leverstein-van Hall, Maurine A] Citation Status MEDLINE |
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| doi: |
10.1371/journal.pone.0052102 |
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| 100 | 1 | |a Voets, Guido M |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Population distribution of Beta-lactamase conferring resistance to third-generation cephalosporins in human clinical Enterobacteriaceae in the Netherlands |
| 264 | 1 | |c 2012 | |
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| 500 | |a Date Completed 25.06.2013 | ||
| 500 | |a Date Revised 21.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a ErratumIn: PLoS One. 2015;9(9):107786 Hall, Maurine A L [corrected to Leverstein-van Hall, Maurine A] | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a There is a global increase in infections caused by Enterobacteriaceae with plasmid-borne β-lactamases that confer resistance to third-generation cephalosporins. The epidemiology of these bacteria is not well understood, and was, therefore, investigated in a selection of 636 clinical Enterobacteriaceae with a minimal inhibitory concentration >1 mg/L for ceftazidime/ceftriaxone from a national survey (75% E. coli, 11% E. cloacae, 11% K. pneumoniae, 2% K. oxytoca, 2% P. mirabilis). Isolates were investigated for extended-spectrum β-lactamases (ESBLs) and ampC genes using microarray, PCR, gene sequencing and molecular straintyping (Diversilab and multi-locus sequence typing (MLST)). ESBL genes were demonstrated in 512 isolates (81%); of which 446 (87%) belonged to the CTX-M family. Among 314 randomly selected and sequenced isolates, bla(CTX-M-15) was most prevalent (n = 124, 39%), followed by bla(CTX-M-1) (n = 47, 15%), bla(CTX-M-14) (n = 15, 5%), bla(SHV-12) (n = 24, 8%) and bla(TEM-52) (n = 13, 4%). Among 181 isolates with MIC ≥16 mg/L for cefoxitin plasmid encoded AmpCs were detected in 32 and 27 were of the CMY-2 group. Among 102 E. coli isolates with MIC ≥16 mg/L for cefoxitin ampC promoter mutations were identified in 29 (28%). Based on Diversilab genotyping of 608 isolates (similarity cut-off >98%) discriminatory indices of bacteria with ESBL and/or ampC genes were 0.994, 0.985 and 0.994 for E. coli, K. pneumoniae and E. cloacae, respectively. Based on similarity cut-off >95% two large clusters of E. coli were apparent (of 43 and 30 isolates) and 21 of 21 that were typed by belonged to ST131 of which 13 contained bla(CTX-M-15). Our findings demonstrate that bla(CTX-M-15) is the most prevalent ESBL and we report a larger than previously reported prevalence of ampC genes among Enterobacteriaceae responsible for resistance to third-generation cephalosporins | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Cephalosporins |2 NLM | |
| 650 | 7 | |a AmpC beta-lactamases |2 NLM | |
| 650 | 7 | |a EC 3.5.2.6 |2 NLM | |
| 650 | 7 | |a beta-Lactamases |2 NLM | |
| 650 | 7 | |a EC 3.5.2.6 |2 NLM | |
| 700 | 1 | |a Platteel, Tamara N |e verfasserin |4 aut | |
| 700 | 1 | |a Fluit, Ad C |e verfasserin |4 aut | |
| 700 | 1 | |a Scharringa, Jelle |e verfasserin |4 aut | |
| 700 | 1 | |a Schapendonk, Claudia M |e verfasserin |4 aut | |
| 700 | 1 | |a Stuart, James Cohen |e verfasserin |4 aut | |
| 700 | 1 | |a Bonten, Marc J M |e verfasserin |4 aut | |
| 700 | 1 | |a Leverstein-van Hall, Maurine A |e verfasserin |4 aut | |
| 700 | 1 | |a Hall, Maurine A L |e verfasserin |4 aut | |
| 700 | 0 | |a National ESBL Surveillance Working Group |e verfasserin |4 aut | |
| 700 | 1 | |a Bonten, M J M |e investigator |4 oth | |
| 700 | 1 | |a van Dam, A P |e investigator |4 oth | |
| 700 | 1 | |a Gasthuis, Onze Lieve Vrouwe |e investigator |4 oth | |
| 700 | 1 | |a Andriesse, G |e investigator |4 oth | |
| 700 | 1 | |a Kluytmans, J A J W |e investigator |4 oth | |
| 700 | 1 | |a Vreede, R W |e investigator |4 oth | |
| 700 | 1 | |a Sebens, F W |e investigator |4 oth | |
| 700 | 1 | |a Sabbe, L J M |e investigator |4 oth | |
| 700 | 1 | |a Schellekens, J F P |e investigator |4 oth | |
| 700 | 1 | |a Vogels, W H M |e investigator |4 oth | |
| 700 | 1 | |a Arends, J P |e investigator |4 oth | |
| 700 | 1 | |a Dorigo-Zetsma, J W |e investigator |4 oth | |
| 700 | 1 | |a Waar, K |e investigator |4 oth | |
| 700 | 1 | |a Vlaminckx, B J M |e investigator |4 oth | |
| 700 | 1 | |a Horrevorts, A M |e investigator |4 oth | |
| 700 | 1 | |a Sturm, P |e investigator |4 oth | |
| 700 | 1 | |a Stals, F S |e investigator |4 oth | |
| 700 | 1 | |a Wintermans, R G F |e investigator |4 oth | |
| 700 | 1 | |a Moffie, B |e investigator |4 oth | |
| 700 | 1 | |a Hendrickx, B G A |e investigator |4 oth | |
| 700 | 1 | |a Buiting, A G M |e investigator |4 oth | |
| 700 | 1 | |a Verhoef, L |e investigator |4 oth | |
| 700 | 1 | |a Tjhie, H T |e investigator |4 oth | |
| 700 | 1 | |a Wolfhagen, M J H M |e investigator |4 oth | |
| 700 | 1 | |a Diederen, B M W |e investigator |4 oth | |
| 700 | 1 | |a Thijssen, S F T |e investigator |4 oth | |
| 700 | 1 | |a Mascini, E M |e investigator |4 oth | |
| 700 | 1 | |a van Griethuysen, A |e investigator |4 oth | |
| 700 | 1 | |a Wever, P C |e investigator |4 oth | |
| 700 | 1 | |a Fleer, A |e investigator |4 oth | |
| 700 | 1 | |a Wagenvoort, J H T |e investigator |4 oth | |
| 700 | 1 | |a Bernards, A T |e investigator |4 oth | |
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