Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus
Copyright © 2012 Elsevier B.V. All rights reserved..
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found ((1)VCKKITEHERCHVNI(15)), ((20)AFFGVMPRGTTPRAPVR(36)), ((46)RRGLETGWAYTHQGGI(61)), ((281)EGDMATGITYASYGYFC(297)), ((426)YSGEDPANLRVVTSQSPYVVVATNAIESGV(455)) and ((481)FIVTGLKRMAVTVGEQA(497)) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody-antigen reaction of the non-structural proteins and may aid in the clinical application as well.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:151 |
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| Enthalten in: |
Veterinary immunology and immunopathology - 151(2013), 3-4 vom: 15. Feb., Seite 331-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Li, Yan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 06.09.2013 Date Revised 21.11.2013 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vetimm.2012.11.011 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM223793035 |
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| 245 | 1 | 0 | |a Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus |
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| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2012 Elsevier B.V. All rights reserved. | ||
| 520 | |a Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found ((1)VCKKITEHERCHVNI(15)), ((20)AFFGVMPRGTTPRAPVR(36)), ((46)RRGLETGWAYTHQGGI(61)), ((281)EGDMATGITYASYGYFC(297)), ((426)YSGEDPANLRVVTSQSPYVVVATNAIESGV(455)) and ((481)FIVTGLKRMAVTVGEQA(497)) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody-antigen reaction of the non-structural proteins and may aid in the clinical application as well | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 650 | 7 | |a Epitopes, B-Lymphocyte |2 NLM | |
| 650 | 7 | |a Peptide Fragments |2 NLM | |
| 650 | 7 | |a Viral Nonstructural Proteins |2 NLM | |
| 650 | 7 | |a p80 protein, bovine viral diarrhea virus |2 NLM | |
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| 650 | 7 | |a Peptide Hydrolases |2 NLM | |
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| 700 | 1 | |a Jia, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Kai |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Mingchun |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wenlong |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Junwei |e verfasserin |4 aut | |
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