Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins
Copyright © 2012 Elsevier B.V. All rights reserved..
Together with reactive oxygen species, nitric oxide is an essential part of the signal transduction induced by stress conditions. In this work we study the pattern of S-nitrosylated proteins from mitochondria of pea plants subjected to 150mM NaCl for 5 and 14days. A differential pattern of target proteins was found during plant development and salt stress, with a minor number of S-nitrosylated proteins at 14 days specifically some key enzymes related to respiration and photorespiration. At this time of stress, only ATP synthase β subunit, peroxiredoxin and Hsp90 were S-nitrosylated and no changes in protein levels were observed, although the activity of PrxII F may be reduced by S-nitrosylation. The NADH/NAD(+) ratio was also high at 14days but not the NADPH/NADP(+). An enhancement in NO measured by fluorimetry and confocal microscopy was observed in leaves, being part of the NO localized in mitochondria. An increase in mitochondrial GSNOR activity was produced in response to short and long-term NaCl treatment, where a higher number of nitrated proteins were also observed. The results indicated that posttranslational modifications seem to modulate respiratory and photorespiratory pathways, as well as some antioxidant enzymes, through differential S-nitrosylation/denitrosylation in control conditions and under salt stress.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2013 |
|---|---|
| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:79 |
|---|---|
| Enthalten in: |
Journal of proteomics - 79(2013) vom: 21. Feb., Seite 87-99 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Camejo, Daymi [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 23.08.2013 Date Revised 09.01.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.jprot.2012.12.003 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM223424951 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM223424951 | ||
| 003 | DE-627 | ||
| 005 | 20240114232604.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231224s2013 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.jprot.2012.12.003 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1253.xml |
| 035 | |a (DE-627)NLM223424951 | ||
| 035 | |a (NLM)23238061 | ||
| 035 | |a (PII)S1874-3919(12)00782-8 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Camejo, Daymi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Salinity-induced changes in S-nitrosylation of pea mitochondrial proteins |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.08.2013 | ||
| 500 | |a Date Revised 09.01.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2012 Elsevier B.V. All rights reserved. | ||
| 520 | |a Together with reactive oxygen species, nitric oxide is an essential part of the signal transduction induced by stress conditions. In this work we study the pattern of S-nitrosylated proteins from mitochondria of pea plants subjected to 150mM NaCl for 5 and 14days. A differential pattern of target proteins was found during plant development and salt stress, with a minor number of S-nitrosylated proteins at 14 days specifically some key enzymes related to respiration and photorespiration. At this time of stress, only ATP synthase β subunit, peroxiredoxin and Hsp90 were S-nitrosylated and no changes in protein levels were observed, although the activity of PrxII F may be reduced by S-nitrosylation. The NADH/NAD(+) ratio was also high at 14days but not the NADPH/NADP(+). An enhancement in NO measured by fluorimetry and confocal microscopy was observed in leaves, being part of the NO localized in mitochondria. An increase in mitochondrial GSNOR activity was produced in response to short and long-term NaCl treatment, where a higher number of nitrated proteins were also observed. The results indicated that posttranslational modifications seem to modulate respiratory and photorespiratory pathways, as well as some antioxidant enzymes, through differential S-nitrosylation/denitrosylation in control conditions and under salt stress | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Mitochondrial Proteins |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
| 650 | 7 | |a Nitric Oxide |2 NLM | |
| 650 | 7 | |a 31C4KY9ESH |2 NLM | |
| 650 | 7 | |a Peroxiredoxins |2 NLM | |
| 650 | 7 | |a EC 1.11.1.15 |2 NLM | |
| 650 | 7 | |a Aldehyde Oxidoreductases |2 NLM | |
| 650 | 7 | |a EC 1.2.- |2 NLM | |
| 650 | 7 | |a formaldehyde dehydrogenase, glutathione-independent |2 NLM | |
| 650 | 7 | |a EC 1.2.1.46 |2 NLM | |
| 700 | 1 | |a Romero-Puertas, María Del Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Serrano, María |e verfasserin |4 aut | |
| 700 | 1 | |a Sandalio, Luisa María |e verfasserin |4 aut | |
| 700 | 1 | |a Lázaro, Juan José |e verfasserin |4 aut | |
| 700 | 1 | |a Jiménez, Ana |e verfasserin |4 aut | |
| 700 | 1 | |a Sevilla, Francisca |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of proteomics |d 2008 |g 79(2013) vom: 21. Feb., Seite 87-99 |w (DE-627)NLM180095633 |x 1876-7737 |7 nnns |
| 773 | 1 | 8 | |g volume:79 |g year:2013 |g day:21 |g month:02 |g pages:87-99 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.jprot.2012.12.003 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 79 |j 2013 |b 21 |c 02 |h 87-99 | ||