Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines
Previous studies have demonstrated that pluripotency-associated transcription factors, such as Oct3/4, Nanog and Sox2, play a crucial role in the development and malignant progression of various types of tumors. Breast cancer is the most frequent cancer among females, being a heterogeneous disease, with distinct morphologies, metastatic behavior and therapeutic responses. The expression of Oct3/4, Nanog and Sox2 in 3 human breast cancer cell lines, MCF7, T-47D and MDA-MB-231, was determined. The expression of Oct3/4, Nanog and Sox2 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR) and protein expression was detected by immunocytohistochemistry. RT-PCR revealed that all three human breast cancer cell lines tested expressed evident Oct3/4, Nanog and Sox-2 mRNA at various levels. Higher levels of Oct3/4 were identified in MCF7 and MDA-MB-231 cells compared with T-47D cells. Higher levels of Nanog were observed in MCF7 and T-47D cells compared with MDA-MB-231 cells and the highest expression of Sox-2 was detected in MCF7 cells. The nuclear localization of Oct3/4, Nanog and Sox-2 was confirmed by immunostaining. Oct3/4, Nanog and Sox2 were expressed in human breast cancer cell lines. Further studies are required to characterize the role of Oct3/4, Nanog and Sox2 in human breast cancer.
Medienart: |
Artikel |
---|
Erscheinungsjahr: |
2012 |
---|---|
Erschienen: |
2012 |
Enthalten in: |
Zur Gesamtaufnahme - volume:4 |
---|---|
Enthalten in: |
Oncology letters - 4(2012), 6 vom: 10. Dez., Seite 1264-1268 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Ling, Gui-Qin [VerfasserIn] |
---|
Themen: |
---|
Anmerkungen: |
Date Revised 21.10.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
---|
Förderinstitution / Projekttitel: |
|
---|
PPN (Katalog-ID): |
NLM223044881 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM223044881 | ||
003 | DE-627 | ||
005 | 20231224055750.0 | ||
007 | tu | ||
008 | 231224s2012 xx ||||| 00| ||eng c | ||
028 | 5 | 2 | |a pubmed24n0743.xml |
035 | |a (DE-627)NLM223044881 | ||
035 | |a (NLM)23197999 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Ling, Gui-Qin |e verfasserin |4 aut | |
245 | 1 | 0 | |a Expression of the pluripotency markers Oct3/4, Nanog and Sox2 in human breast cancer cell lines |
264 | 1 | |c 2012 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a Date Revised 21.10.2021 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Previous studies have demonstrated that pluripotency-associated transcription factors, such as Oct3/4, Nanog and Sox2, play a crucial role in the development and malignant progression of various types of tumors. Breast cancer is the most frequent cancer among females, being a heterogeneous disease, with distinct morphologies, metastatic behavior and therapeutic responses. The expression of Oct3/4, Nanog and Sox2 in 3 human breast cancer cell lines, MCF7, T-47D and MDA-MB-231, was determined. The expression of Oct3/4, Nanog and Sox2 mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR) and protein expression was detected by immunocytohistochemistry. RT-PCR revealed that all three human breast cancer cell lines tested expressed evident Oct3/4, Nanog and Sox-2 mRNA at various levels. Higher levels of Oct3/4 were identified in MCF7 and MDA-MB-231 cells compared with T-47D cells. Higher levels of Nanog were observed in MCF7 and T-47D cells compared with MDA-MB-231 cells and the highest expression of Sox-2 was detected in MCF7 cells. The nuclear localization of Oct3/4, Nanog and Sox-2 was confirmed by immunostaining. Oct3/4, Nanog and Sox2 were expressed in human breast cancer cell lines. Further studies are required to characterize the role of Oct3/4, Nanog and Sox2 in human breast cancer | ||
650 | 4 | |a Journal Article | |
700 | 1 | |a Chen, Dong-Bo |e verfasserin |4 aut | |
700 | 1 | |a Wang, Bao-Qing |e verfasserin |4 aut | |
700 | 1 | |a Zhang, Lan-Sheng |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Oncology letters |d 2010 |g 4(2012), 6 vom: 10. Dez., Seite 1264-1268 |w (DE-627)NLM201171317 |x 1792-1082 |7 nnns |
773 | 1 | 8 | |g volume:4 |g year:2012 |g number:6 |g day:10 |g month:12 |g pages:1264-1268 |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 4 |j 2012 |e 6 |b 10 |c 12 |h 1264-1268 |