Continuous labeling of circulating tumor cells with microbeads using a vortex micromixer for highly selective isolation
Copyright © 2012 Elsevier B.V. All rights reserved..
Circulating tumor cells (CTCs) are identified in transit within the blood stream of cancer patients and have been proven to be a main cause of metastatic disease. Current approaches for the size-based isolation of CTCs have encountered technical challenges as some of the CTCs have a size similar to that of leukocytes and therefore CTCs are often lost in the process. Here, we propose a novel strategy where most of the CTCs are coated by a large number of microbeads to amplify their size to enable complete discrimination from leukocytes. In addition, all of the microbead labeling processes are carried out in a continuous manner to prevent any loss of CTCs during the isolation process. Thus, a microfluidic mixer was employed to facilitate the efficient and selective labeling of CTCs from peripheral blood samples. By generating secondary vortex flows called Taylor-Gortler vortices perpendicular to the main flow direction in our microfluidic device, CTCs were continuously and successfully coated with anti-epithelial cell adhesion molecule-conjugated beads. After the continuous labeling, the enlarged CTCs were perfectly trapped in a micro-filter whereas all of the leukocytes escaped.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2013 |
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| Erschienen: |
2013 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:40 |
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| Enthalten in: |
Biosensors & bioelectronics - 40(2013), 1 vom: 15. Feb., Seite 63-7 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lin, Ming Xian [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 25.04.2013 Date Revised 12.11.2012 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bios.2012.06.016 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM219338957 |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2012 Elsevier B.V. All rights reserved. | ||
| 520 | |a Circulating tumor cells (CTCs) are identified in transit within the blood stream of cancer patients and have been proven to be a main cause of metastatic disease. Current approaches for the size-based isolation of CTCs have encountered technical challenges as some of the CTCs have a size similar to that of leukocytes and therefore CTCs are often lost in the process. Here, we propose a novel strategy where most of the CTCs are coated by a large number of microbeads to amplify their size to enable complete discrimination from leukocytes. In addition, all of the microbead labeling processes are carried out in a continuous manner to prevent any loss of CTCs during the isolation process. Thus, a microfluidic mixer was employed to facilitate the efficient and selective labeling of CTCs from peripheral blood samples. By generating secondary vortex flows called Taylor-Gortler vortices perpendicular to the main flow direction in our microfluidic device, CTCs were continuously and successfully coated with anti-epithelial cell adhesion molecule-conjugated beads. After the continuous labeling, the enlarged CTCs were perfectly trapped in a micro-filter whereas all of the leukocytes escaped | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 700 | 1 | |a Hyun, Kyung-A |e verfasserin |4 aut | |
| 700 | 1 | |a Moon, Hui-Sung |e verfasserin |4 aut | |
| 700 | 1 | |a Sim, Tae Seok |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Jeong-Gun |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Jae Chan |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Soo Suk |e verfasserin |4 aut | |
| 700 | 1 | |a Jung, Hyo-Il |e verfasserin |4 aut | |
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