Cytogenetic analysis of childhood acute lymphoblastic leukemia
OBJECTIVE: To characterize the genetic aberrations in pediatric acute lymphoblastic leukemia (ALL).
METHODS: Ninety ALL cases were enrolled in the study from January 2009 to November 2011. Chromosome banding analysis and fluorescence in situ hybridization (FISH) were used to detect genetic aberrations.
RESULTS: (1) Chromosome analysis: 35 (53.0%) of 66 cases who had metaphase were abnormal, and 24 cases had no metaphase. (2) FISH analysis: among the 31 cases who had normal karyotypes and 24 who had no metaphase detected by chromosome banding technique, 7 (22.6%) and 14 (58.3%) cases were abnormal detected by FISH, respectively. There were no statistically significant differences compared with chromosome analysis (P = 0.655). Among these 55 ALL cases TEL/AML1, bcr-abl and MLL fusion genes were observed in 16 (29.1%), 3(5.5%) and 2(3.6%) cases, respectively. (3) Cytogenetic aberration was observed in 56 of total 90 ALL cases (62.2%).
CONCLUSIONS: Cytogenetic changes are common in childhood ALL. Conventional cytogenetic study could reliably detected chromosomal abnormalities for ALL with assessable metaphase. FISH should be used as a complementary method for ALL patients who have poor chromosomal morphology or no metaphase cells, and combination of both methods can improve the detection rate of genetic abnormalities in childhood leukemia.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2012 |
|---|---|
| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
|---|---|
| Enthalten in: |
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 33(2012), 4 vom: 04. Apr., Seite 282-5 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Liu, Qing [VerfasserIn] |
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| Themen: |
149025-06-9 |
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| Anmerkungen: |
Date Completed 29.08.2013 Date Revised 21.11.2013 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM219313458 |
|---|
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| 100 | 1 | |a Liu, Qing |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Cytogenetic analysis of childhood acute lymphoblastic leukemia |
| 264 | 1 | |c 2012 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 29.08.2013 | ||
| 500 | |a Date Revised 21.11.2013 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To characterize the genetic aberrations in pediatric acute lymphoblastic leukemia (ALL) | ||
| 520 | |a METHODS: Ninety ALL cases were enrolled in the study from January 2009 to November 2011. Chromosome banding analysis and fluorescence in situ hybridization (FISH) were used to detect genetic aberrations | ||
| 520 | |a RESULTS: (1) Chromosome analysis: 35 (53.0%) of 66 cases who had metaphase were abnormal, and 24 cases had no metaphase. (2) FISH analysis: among the 31 cases who had normal karyotypes and 24 who had no metaphase detected by chromosome banding technique, 7 (22.6%) and 14 (58.3%) cases were abnormal detected by FISH, respectively. There were no statistically significant differences compared with chromosome analysis (P = 0.655). Among these 55 ALL cases TEL/AML1, bcr-abl and MLL fusion genes were observed in 16 (29.1%), 3(5.5%) and 2(3.6%) cases, respectively. (3) Cytogenetic aberration was observed in 56 of total 90 ALL cases (62.2%) | ||
| 520 | |a CONCLUSIONS: Cytogenetic changes are common in childhood ALL. Conventional cytogenetic study could reliably detected chromosomal abnormalities for ALL with assessable metaphase. FISH should be used as a complementary method for ALL patients who have poor chromosomal morphology or no metaphase cells, and combination of both methods can improve the detection rate of genetic abnormalities in childhood leukemia | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Myeloid-Lymphoid Leukemia Protein |2 NLM | |
| 650 | 7 | |a 149025-06-9 |2 NLM | |
| 650 | 7 | |a Fusion Proteins, bcr-abl |2 NLM | |
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| 700 | 1 | |a Jiang, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Heng-juan |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Yi-ju |e verfasserin |4 aut | |
| 700 | 1 | |a Bao, Li-ming |e verfasserin |4 aut | |
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