Determination of ebselen-sensitive reactive oxygen metabolites (ebROM) in human serum based upon N,N'-diethyl-1,4-phenylenediamine oxidation
Copyright © 2012 Elsevier B.V. All rights reserved..
BACKGROUND: Oxidative stress occurs through free radical- and non-radical-mediated oxidative mechanisms, but these are poorly discriminated by most assays. A convenient assay for oxidants in human serum is based upon the Fe(2+)-dependent decomposition of peroxides to oxidize N,N'-diethyl-1,4-phenylenediamine (DEPPD) to a stable radical cation which can be measured spectrophotometrically.
METHODS: We investigated modification of the DEPPD oxidation assay to discriminate color formation due to non-radical oxidants, including hydroperoxides and endoperoxides, which are sensitive to ebselen.
RESULTS: Use of serum, which has been pretreated with ebselen as a reference, provides a quantitative assay for non-radical, reactive oxidant species in serum, including hydroperoxides, endoperoxides and epoxides. In a set of 35 human serum samples, non-radical oxidants largely accounted for DEPPD oxidation in 86% of the samples while the remaining 14% had considerable contribution from other redox-active chemicals.
CONCLUSIONS: The simple modification in which ebselen-pretreated sample is used as a reference provides means to quantify non-radical oxidants in human serum. Application of this approach could enhance understanding of the contribution of different types of oxidative stress to disease.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:414 |
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| Enthalten in: |
Clinica chimica acta; international journal of clinical chemistry - 414(2012) vom: 24. Dez., Seite 1-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liang, Yongliang [VerfasserIn] |
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| Links: |
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| Themen: |
40X2P7DPGH |
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| Anmerkungen: |
Date Completed 17.05.2013 Date Revised 03.12.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.cca.2012.06.003 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM218527985 |
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| 041 | |a eng | ||
| 100 | 1 | |a Liang, Yongliang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Determination of ebselen-sensitive reactive oxygen metabolites (ebROM) in human serum based upon N,N'-diethyl-1,4-phenylenediamine oxidation |
| 264 | 1 | |c 2012 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 17.05.2013 | ||
| 500 | |a Date Revised 03.12.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2012 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: Oxidative stress occurs through free radical- and non-radical-mediated oxidative mechanisms, but these are poorly discriminated by most assays. A convenient assay for oxidants in human serum is based upon the Fe(2+)-dependent decomposition of peroxides to oxidize N,N'-diethyl-1,4-phenylenediamine (DEPPD) to a stable radical cation which can be measured spectrophotometrically | ||
| 520 | |a METHODS: We investigated modification of the DEPPD oxidation assay to discriminate color formation due to non-radical oxidants, including hydroperoxides and endoperoxides, which are sensitive to ebselen | ||
| 520 | |a RESULTS: Use of serum, which has been pretreated with ebselen as a reference, provides a quantitative assay for non-radical, reactive oxidant species in serum, including hydroperoxides, endoperoxides and epoxides. In a set of 35 human serum samples, non-radical oxidants largely accounted for DEPPD oxidation in 86% of the samples while the remaining 14% had considerable contribution from other redox-active chemicals | ||
| 520 | |a CONCLUSIONS: The simple modification in which ebselen-pretreated sample is used as a reference provides means to quantify non-radical oxidants in human serum. Application of this approach could enhance understanding of the contribution of different types of oxidative stress to disease | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Azoles |2 NLM | |
| 650 | 7 | |a Isoindoles |2 NLM | |
| 650 | 7 | |a Organoselenium Compounds |2 NLM | |
| 650 | 7 | |a Phenylenediamines |2 NLM | |
| 650 | 7 | |a ebselen |2 NLM | |
| 650 | 7 | |a 40X2P7DPGH |2 NLM | |
| 650 | 7 | |a Oxygen |2 NLM | |
| 650 | 7 | |a S88TT14065 |2 NLM | |
| 700 | 1 | |a Roede, James R |e verfasserin |4 aut | |
| 700 | 1 | |a Dikalov, Sergey |e verfasserin |4 aut | |
| 700 | 1 | |a Miller, Nana Gletsu |e verfasserin |4 aut | |
| 700 | 1 | |a Dudley, Samuel C |c Jr |e verfasserin |4 aut | |
| 700 | 1 | |a Quyyumi, Arshed |e verfasserin |4 aut | |
| 700 | 1 | |a Jones, Dean P |e verfasserin |4 aut | |
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