Apigenin regulates lipopolysaccharides-induced activation of inflammasome
OBJECTIVE: To evaluate whether or not apigenin regulates the activation of inflammasome and elucidate its underlying mechanism.
METHODS: Cultured THP-1 (acute monocytic leukemia cell line) cells were treated with dimethyl sulfoxide (DMSO) alone (control group), lipopolysaccharides (LPS), LPS plus apigenin (25/50 µmol/L) or LPS plus Z-VAD (a caspase inhibitor). The supernatant was harvested and the content of secreted interleukin (IL)-1β was determined by enzyme-linked immunosorbent assay (ELISA). The effects of apigenin on the cleavage of pro-IL-1β and pro-caspase-1 were determined by Western blot. And the effect of apigenin on the nuclear factor (NF)-κB activation was detected by reporter gene assay.
RESULTS: MTT assays showed that the cytotoxicity of apigenin was low. Apigenin could significantly inhibit the LPS-induced secretion of IL-1β in THP-1 cells. The concentration of IL-1β was (362 ± 64) pg/ml in the control group, (1549 ± 320) pg/ml in the LPS group, (397 ± 150) pg/ml in the LPS plus 25 µmol/L apigenin group and (268 ± 142) pg/ml in the LPS plus 50 µmol/L apigenin group (P < 0.05). The results of Western blot indicated that apigenin inhibited the maturation of pro-IL-1β and pro-caspase-1. It could also inhibit the LPS-induced activation of NF-κB. The value of relative light unit was 0.6 ± 0.1 in the control group, 32.7 ± 0.8 in the LPS group, 12.9 ± 1.8 in the LPS plus 25 µmol/L apigenin group and 10.0 ± 3.2 in the LPS plus 50 µmol/L apigenin group respectively (P < 0.05).
CONCLUSION: Apigenin may inhibit the LPS-induced activation of inflammasome through an inhibited muturation of caspase-1.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:91 |
|---|---|
| Enthalten in: |
Zhonghua yi xue za zhi - 91(2011), 34 vom: 13. Sept., Seite 2435-9 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Wang, Ling-Yan [VerfasserIn] |
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| Themen: |
7V515PI7F6 |
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| Anmerkungen: |
Date Completed 23.04.2016 Date Revised 01.12.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM21529601X |
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| 500 | |a Date Completed 23.04.2016 | ||
| 500 | |a Date Revised 01.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To evaluate whether or not apigenin regulates the activation of inflammasome and elucidate its underlying mechanism | ||
| 520 | |a METHODS: Cultured THP-1 (acute monocytic leukemia cell line) cells were treated with dimethyl sulfoxide (DMSO) alone (control group), lipopolysaccharides (LPS), LPS plus apigenin (25/50 µmol/L) or LPS plus Z-VAD (a caspase inhibitor). The supernatant was harvested and the content of secreted interleukin (IL)-1β was determined by enzyme-linked immunosorbent assay (ELISA). The effects of apigenin on the cleavage of pro-IL-1β and pro-caspase-1 were determined by Western blot. And the effect of apigenin on the nuclear factor (NF)-κB activation was detected by reporter gene assay | ||
| 520 | |a RESULTS: MTT assays showed that the cytotoxicity of apigenin was low. Apigenin could significantly inhibit the LPS-induced secretion of IL-1β in THP-1 cells. The concentration of IL-1β was (362 ± 64) pg/ml in the control group, (1549 ± 320) pg/ml in the LPS group, (397 ± 150) pg/ml in the LPS plus 25 µmol/L apigenin group and (268 ± 142) pg/ml in the LPS plus 50 µmol/L apigenin group (P < 0.05). The results of Western blot indicated that apigenin inhibited the maturation of pro-IL-1β and pro-caspase-1. It could also inhibit the LPS-induced activation of NF-κB. The value of relative light unit was 0.6 ± 0.1 in the control group, 32.7 ± 0.8 in the LPS group, 12.9 ± 1.8 in the LPS plus 25 µmol/L apigenin group and 10.0 ± 3.2 in the LPS plus 50 µmol/L apigenin group respectively (P < 0.05) | ||
| 520 | |a CONCLUSION: Apigenin may inhibit the LPS-induced activation of inflammasome through an inhibited muturation of caspase-1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Inflammasomes |2 NLM | |
| 650 | 7 | |a Interleukin-1beta |2 NLM | |
| 650 | 7 | |a Lipopolysaccharides |2 NLM | |
| 650 | 7 | |a NF-kappa B |2 NLM | |
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| 700 | 1 | |a Kuang, Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jing |e verfasserin |4 aut | |
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