Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment
A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
|---|---|
| Enthalten in: |
Journal of clinical microbiology - 50(2012), 4 vom: 01. Apr., Seite 1412-4 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
McConnell, Michael J [VerfasserIn] |
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| Links: |
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| Themen: |
149024-69-1 |
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| Anmerkungen: |
Date Completed 10.07.2012 Date Revised 21.10.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1128/JCM.06566-11 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM215095979 |
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| 245 | 1 | 0 | |a Quantitative real-time PCR for detection of Acinetobacter baumannii colonization in the hospital environment |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Bacterial Outer Membrane Proteins |2 NLM | |
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| 700 | 1 | |a Pérez-Romero, Pilar |e verfasserin |4 aut | |
| 700 | 1 | |a Valencia, Raquel |e verfasserin |4 aut | |
| 700 | 1 | |a Lepe, José Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Vázquez-Barba, Isabel |e verfasserin |4 aut | |
| 700 | 1 | |a Pachón, Jerónimo |e verfasserin |4 aut | |
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