Dielectrophoresis and shear-enhanced sensitivity and selectivity of DNA hybridization for the rapid discrimination of Candida species
Copyright © 2011 Elsevier B.V. All rights reserved..
We present a dielectrophoresis (DEP)-based microfluidic chip that is capable of enhancing the sensitivity and selectivity of DNA hybridization using an AC electric field and hydrodynamic shear in a continuous through-flow. Molecular DEP was employed to rapidly trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a microfluidic chip with a locally amplified AC electric field gradient. The detection time can be accelerated to sub-minute periods, and the sensitivity can reach the pico-molar level due to the AC DEP-enhanced molecule concentration (at an optimal AC frequency of 900 kHz) in a small region (∼100 μm(2)) instead of the broad area used in a tank reactor (∼10(6) μm(2)). Continuous flow in a microchannel provides a constant and high shear rate that can shear off most non-specific target-probe binding to promote the discriminating selectivity. On-chip multi-target discrimination of Candida species can be achieved within a few minutes under optimal conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Biosensors & bioelectronics - 33(2012), 1 vom: 15. März, Seite 36-43 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cheng, I-Fang [VerfasserIn] |
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| Anmerkungen: |
Date Completed 21.06.2012 Date Revised 27.02.2012 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bios.2011.12.005 |
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| Förderinstitution / Projekttitel: |
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NLM214420701 |
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| 500 | |a Date Revised 27.02.2012 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2011 Elsevier B.V. All rights reserved. | ||
| 520 | |a We present a dielectrophoresis (DEP)-based microfluidic chip that is capable of enhancing the sensitivity and selectivity of DNA hybridization using an AC electric field and hydrodynamic shear in a continuous through-flow. Molecular DEP was employed to rapidly trap ssDNA molecules in a flowing solution to a cusp-shaped nanocolloid assembly on a microfluidic chip with a locally amplified AC electric field gradient. The detection time can be accelerated to sub-minute periods, and the sensitivity can reach the pico-molar level due to the AC DEP-enhanced molecule concentration (at an optimal AC frequency of 900 kHz) in a small region (∼100 μm(2)) instead of the broad area used in a tank reactor (∼10(6) μm(2)). Continuous flow in a microchannel provides a constant and high shear rate that can shear off most non-specific target-probe binding to promote the discriminating selectivity. On-chip multi-target discrimination of Candida species can be achieved within a few minutes under optimal conditions | ||
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| 700 | 1 | |a Chang, Hsien-Chang |e verfasserin |4 aut | |
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