Localization of nerve growth factor (NGF) receptors in the mitochondrial compartment : characterization and putative role
Copyright © 2011 Elsevier B.V. All rights reserved..
BACKGROUND: The neurotrophin NGF receptors trkA and p75NTR are expressed in the central and peripheral nervous system as well as in non-neuronal tissues; originally described to localize to the plasma membrane, recent studies have suggested other intracellular localizations for both NGF receptors.
SCOPE OF REVIEW: In order to determine whether NGF receptors localize to the mitochondrial compartment mitochondria isolated from human kidney, rat tissues and a human podocyte as cell line before and after differentiation were used.
MAJOR CONCLUSIONS: Our results demonstrate that NGF receptors are localized in the mitochondrial compartment of undifferentiated human podocytes and in all tissues analyzed including rat central nervous system. In mitochondria p75NTR, but not trkA, co-immunoprecipitates with the adenine nucleotide translocator (ANT) and the phosphodiesterase 4 isoform A5 (PDE4A5). Moreover, NGF, via trkA, protects isolated mitochondria of rat brain cortex from mitochondrial permeability transition induced by Ca(2+).
GENERAL SIGNIFICANCE: Although NGF receptors have been described as mainly citoplasmatic so far, we proved evidence of their expression at the mitochondrial level and their interaction with specific proteins. Our results demonstrating the expression of NGF receptors in the mitochondria provide new insights into the role of NGF at subcellular level, in different areas of the organism, including CNS.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2012 |
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| Erschienen: |
2012 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1820 |
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| Enthalten in: |
Biochimica et biophysica acta - 1820(2012), 2 vom: 15. Feb., Seite 96-103 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Carito, Valentina [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 10.04.2012 Date Revised 26.11.2016 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bbagen.2011.10.015 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM213568004 |
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| 041 | |a eng | ||
| 100 | 1 | |a Carito, Valentina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Localization of nerve growth factor (NGF) receptors in the mitochondrial compartment |b characterization and putative role |
| 264 | 1 | |c 2012 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 10.04.2012 | ||
| 500 | |a Date Revised 26.11.2016 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2011 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: The neurotrophin NGF receptors trkA and p75NTR are expressed in the central and peripheral nervous system as well as in non-neuronal tissues; originally described to localize to the plasma membrane, recent studies have suggested other intracellular localizations for both NGF receptors | ||
| 520 | |a SCOPE OF REVIEW: In order to determine whether NGF receptors localize to the mitochondrial compartment mitochondria isolated from human kidney, rat tissues and a human podocyte as cell line before and after differentiation were used | ||
| 520 | |a MAJOR CONCLUSIONS: Our results demonstrate that NGF receptors are localized in the mitochondrial compartment of undifferentiated human podocytes and in all tissues analyzed including rat central nervous system. In mitochondria p75NTR, but not trkA, co-immunoprecipitates with the adenine nucleotide translocator (ANT) and the phosphodiesterase 4 isoform A5 (PDE4A5). Moreover, NGF, via trkA, protects isolated mitochondria of rat brain cortex from mitochondrial permeability transition induced by Ca(2+) | ||
| 520 | |a GENERAL SIGNIFICANCE: Although NGF receptors have been described as mainly citoplasmatic so far, we proved evidence of their expression at the mitochondrial level and their interaction with specific proteins. Our results demonstrating the expression of NGF receptors in the mitochondria provide new insights into the role of NGF at subcellular level, in different areas of the organism, including CNS | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Adenine Nucleotide Translocator 1 |2 NLM | |
| 650 | 7 | |a Receptors, Nerve Growth Factor |2 NLM | |
| 650 | 7 | |a Receptor, trkA |2 NLM | |
| 650 | 7 | |a EC 2.7.10.1 |2 NLM | |
| 650 | 7 | |a Extracellular Signal-Regulated MAP Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.11.24 |2 NLM | |
| 650 | 7 | |a p38 Mitogen-Activated Protein Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.11.24 |2 NLM | |
| 650 | 7 | |a Cyclic Nucleotide Phosphodiesterases, Type 4 |2 NLM | |
| 650 | 7 | |a EC 3.1.4.17 |2 NLM | |
| 650 | 7 | |a Calcium |2 NLM | |
| 650 | 7 | |a SY7Q814VUP |2 NLM | |
| 700 | 1 | |a Pingitore, Attilio |e verfasserin |4 aut | |
| 700 | 1 | |a Cione, Erika |e verfasserin |4 aut | |
| 700 | 1 | |a Perrotta, Ida |e verfasserin |4 aut | |
| 700 | 1 | |a Mancuso, Domenico |e verfasserin |4 aut | |
| 700 | 1 | |a Russo, Antonio |e verfasserin |4 aut | |
| 700 | 1 | |a Genchi, Giuseppe |e verfasserin |4 aut | |
| 700 | 1 | |a Caroleo, Maria Cristina |e verfasserin |4 aut | |
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