Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen-Friedenreich antigen-specific Amaranthus caudatus lectin
Copyright © 2011 Elsevier B.V. All rights reserved..
The reactivity of different lectins with crude chromogranin A (CgA) obtained from different animals, namely, cow, horse, dog, pig, and dolphin, was examined to identify lectin(s) that would be useful as coating reagent(s) in a sandwich enzyme-linked immunosorbent assay (ELISA). Of the different lectins studied, the Amaranthus caudatus lectin (ACA), which is specific for the Thomsen-Friedenreich (T)-antigen (Galβ1-3GalNAc), was found to react with the CgA from different animals by western blotting. Purified rabbit anti-bovine CgA antibody was also found to cross-react with the crude CgA preparations. On the basis of these findings, a sandwich ELISA was developed with ACA as the coating reagent and anti-bovine CgA antibody as the probing antibody. Using this method, concentration-dependent curves ranging from 0.003 μg/mL to 25 μg/mL and from 0.02 μg/mL to 25 μg/mL were obtained for bovine CgA and canine CgA, respectively. Similarly, concentration-dependent curves were obtained for the equine, swine, and dolphin crude CgA extracts. Thus, ACA is concluded to be a valuable reagent for CgA detection in crude extracts from different animal species, and for CgA isolation/purification.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:144 |
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| Enthalten in: |
Veterinary immunology and immunopathology - 144(2011), 3-4 vom: 15. Dez., Seite 255-8 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Akiyoshi, Hideo [VerfasserIn] |
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| Links: |
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| Themen: |
3554-90-3 |
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| Anmerkungen: |
Date Completed 09.03.2012 Date Revised 21.11.2011 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.vetimm.2011.08.024 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM212637347 |
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| 245 | 1 | 0 | |a Detection of chromogranin A in the adrenal gland extracts of different animal species by an enzyme-linked immunosorbent assay using Thomsen-Friedenreich antigen-specific Amaranthus caudatus lectin |
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| 520 | |a Copyright © 2011 Elsevier B.V. All rights reserved. | ||
| 520 | |a The reactivity of different lectins with crude chromogranin A (CgA) obtained from different animals, namely, cow, horse, dog, pig, and dolphin, was examined to identify lectin(s) that would be useful as coating reagent(s) in a sandwich enzyme-linked immunosorbent assay (ELISA). Of the different lectins studied, the Amaranthus caudatus lectin (ACA), which is specific for the Thomsen-Friedenreich (T)-antigen (Galβ1-3GalNAc), was found to react with the CgA from different animals by western blotting. Purified rabbit anti-bovine CgA antibody was also found to cross-react with the crude CgA preparations. On the basis of these findings, a sandwich ELISA was developed with ACA as the coating reagent and anti-bovine CgA antibody as the probing antibody. Using this method, concentration-dependent curves ranging from 0.003 μg/mL to 25 μg/mL and from 0.02 μg/mL to 25 μg/mL were obtained for bovine CgA and canine CgA, respectively. Similarly, concentration-dependent curves were obtained for the equine, swine, and dolphin crude CgA extracts. Thus, ACA is concluded to be a valuable reagent for CgA detection in crude extracts from different animal species, and for CgA isolation/purification | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Antigens, Tumor-Associated, Carbohydrate |2 NLM | |
| 650 | 7 | |a Chromogranin A |2 NLM | |
| 650 | 7 | |a Plant Lectins |2 NLM | |
| 650 | 7 | |a Tissue Extracts |2 NLM | |
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| 650 | 7 | |a 3554-90-3 |2 NLM | |
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| 700 | 1 | |a Tanaka, Toshiyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Cao |e verfasserin |4 aut | |
| 700 | 1 | |a Yijyun, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Aoki, Mica |e verfasserin |4 aut | |
| 700 | 1 | |a Takenaka, Shigeo |e verfasserin |4 aut | |
| 700 | 1 | |a Shimada, Terumasa |e verfasserin |4 aut | |
| 700 | 1 | |a Shimizu, Junichiro |e verfasserin |4 aut | |
| 700 | 1 | |a Kiyomiya, Ken-Ichi |e verfasserin |4 aut | |
| 700 | 1 | |a Ohashi, Fumihito |e verfasserin |4 aut | |
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