Periodontal ligament regeneration using apical tooth germ cell-conditioned medium induced periodontal ligament cells sheet between dental tube and ceramic biologic bone
OBJECTIVE: The purpose of this study was to establish an indirect co-culture system of rat apical tooth germ-conditioned medium (APTG-CM) and periodontal ligament cells (PDLCs).
METHODS: PDLCs were isolated and cultured through the method of enzyme-digestion. Vimentin and cytokeratin(CK) were used to demonstrate the cells' mesenchymal derivation. Co-culture system of APTG-CM and PDLCs for 28 days, osteocalcin (OCN), collagen type I (COL I) and bone sialoprotein (BSP) were detected in PDLCs by immunocytochemistry. Morphological changes were observed by inverted microscope. With building a transplant by dental tube, periodontal ligament cell sheet and ceramic biologic bone (CBB) in vitro, then, the combinations of dental tube and PDLCs incubated by APTG-CM were implanted subcutaneously into athymic mice for 8 weeks.
RESULTS: This study demonstrated that cellular cementum-like tissue formed along the dentin surface and CBB, with fibrous tissue adjacent or inserted into CBB in vivo. PDLCs were grown better in the CBB than in dentin tubes. And the vertical fibers can't embed in the control.
CONCLUSION: PDLCs, embedded within this APTG-CM, exhibite several phenotypic characteristics of cementoblast lineages. Thereby it contributes to the main processes of periodontal tissue regeneration with rat APTG-CM.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2011 |
|---|---|
| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
|---|---|
| Enthalten in: |
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology - 29(2011), 4 vom: 08. Aug., Seite 429-33 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Ning, Huiying [VerfasserIn] |
|---|
| Themen: |
104982-03-8 |
|---|
| Anmerkungen: |
Date Completed 07.06.2015 Date Revised 01.12.2018 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM211611719 |
|---|
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| 100 | 1 | |a Ning, Huiying |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Periodontal ligament regeneration using apical tooth germ cell-conditioned medium induced periodontal ligament cells sheet between dental tube and ceramic biologic bone |
| 264 | 1 | |c 2011 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 07.06.2015 | ||
| 500 | |a Date Revised 01.12.2018 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: The purpose of this study was to establish an indirect co-culture system of rat apical tooth germ-conditioned medium (APTG-CM) and periodontal ligament cells (PDLCs) | ||
| 520 | |a METHODS: PDLCs were isolated and cultured through the method of enzyme-digestion. Vimentin and cytokeratin(CK) were used to demonstrate the cells' mesenchymal derivation. Co-culture system of APTG-CM and PDLCs for 28 days, osteocalcin (OCN), collagen type I (COL I) and bone sialoprotein (BSP) were detected in PDLCs by immunocytochemistry. Morphological changes were observed by inverted microscope. With building a transplant by dental tube, periodontal ligament cell sheet and ceramic biologic bone (CBB) in vitro, then, the combinations of dental tube and PDLCs incubated by APTG-CM were implanted subcutaneously into athymic mice for 8 weeks | ||
| 520 | |a RESULTS: This study demonstrated that cellular cementum-like tissue formed along the dentin surface and CBB, with fibrous tissue adjacent or inserted into CBB in vivo. PDLCs were grown better in the CBB than in dentin tubes. And the vertical fibers can't embed in the control | ||
| 520 | |a CONCLUSION: PDLCs, embedded within this APTG-CM, exhibite several phenotypic characteristics of cementoblast lineages. Thereby it contributes to the main processes of periodontal tissue regeneration with rat APTG-CM | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Biological Products |2 NLM | |
| 650 | 7 | |a Culture Media, Conditioned |2 NLM | |
| 650 | 7 | |a Osteocalcin |2 NLM | |
| 650 | 7 | |a 104982-03-8 |2 NLM | |
| 700 | 1 | |a Liu, Hongwei |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology |d 1997 |g 29(2011), 4 vom: 08. Aug., Seite 429-33 |w (DE-627)NLM113810970 |x 2618-0456 |7 nnns |
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