DNA methylation of microRNA-34b/c and microRNA-124a in gastric cancer
OBJECTIVE: To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma.
METHODS: A total of 80 subjects were divided into gastric carcinoma group (n = 40) and control group (n = 40). The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues.
RESULTS: The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5% (31/40) and 5.0% (2/40) in gastric carcinoma and control groups respectively. There was statistically significant difference between two groups (P < 0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0% (24/40) and 0 in these two groups respectively. There was statistically significant difference between two groups (P < 0.05). Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma.
CONCLUSION: The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:91 |
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| Enthalten in: |
Zhonghua yi xue za zhi - 91(2011), 23 vom: 21. Juni, Seite 1640-2 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Li, Yang [VerfasserIn] |
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| Themen: |
English Abstract |
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| Anmerkungen: |
Date Completed 30.04.2012 Date Revised 14.09.2011 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM211440604 |
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| 100 | 1 | |a Li, Yang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a DNA methylation of microRNA-34b/c and microRNA-124a in gastric cancer |
| 264 | 1 | |c 2011 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 30.04.2012 | ||
| 500 | |a Date Revised 14.09.2011 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To explore the effects of DNA methylation of microRNA (miRNA) gene on their expressions in patients with gastric carcinoma | ||
| 520 | |a METHODS: A total of 80 subjects were divided into gastric carcinoma group (n = 40) and control group (n = 40). The DNA methylation status of miRNA-34b/c and miRNA-124a gene promoters was detected by DNA methylation specific polymerase chain reaction (MSP) in gastric carcinoma tissues and normal mucosal tissues | ||
| 520 | |a RESULTS: The positive rate of DNA methylation of miRNA-34b/c gene promoter was 77.5% (31/40) and 5.0% (2/40) in gastric carcinoma and control groups respectively. There was statistically significant difference between two groups (P < 0.05). The positive rate of DNA methylation of miRNA-124a gene promoter was 60.0% (24/40) and 0 in these two groups respectively. There was statistically significant difference between two groups (P < 0.05). Also the hypermethylation positivity of gene promoter of miRNAs was correlated with the clinicopathological features of gastric carcinoma | ||
| 520 | |a CONCLUSION: The hypermethylation of miRNA-34b/c and miRNA-124a gene promoters may play a crucial role in the occurrence and development of gastric carcinoma | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a MIRN124 microRNA, human |2 NLM | |
| 650 | 7 | |a MIRN34 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a Yue, Hong-yu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wen-tian |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Wei-li |e verfasserin |4 aut | |
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