Diversity of acetic acid bacteria present in healthy grapes from the Canary Islands
Copyright © 2011 Elsevier B.V. All rights reserved..
The identification of acetic acid bacteria (AAB) from sound grapes from the Canary Islands is reported in the present study. No direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from Tenerife, La Palma and Lanzarote islands. Up to 396 AAB were isolated from those microvinifications and identified by 16S rRNA gene sequencing and phylogenetic analysis. With this method, Acetobacter pasteurianus, Acetobacter tropicalis, Gluconobacter japonicus and Gluconacetobacter saccharivorans were identified. However, no discrimination between the closely related species Acetobacter malorum and Acetobacter cerevisiae was possible. As previously described, 16S-23S rRNA gene internal transcribed spacer (ITS) region phylogenetic analysis was required to classify isolates as one of those species. These two species were the most frequently occurring, accounting for more than 60% of the isolates. For typing the AAB isolates, both the Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR and (GTG)5-PCR techniques gave similar resolution. A total of 60 profiles were identified. Thirteen of these profiles were found in more than one vineyard, and only one profile was found on two different islands (Tenerife and La Palma).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:151 |
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| Enthalten in: |
International journal of food microbiology - 151(2011), 1 vom: 15. Nov., Seite 105-12 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Valera, Maria José [VerfasserIn] |
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| Links: |
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| Themen: |
DNA, Ribosomal Spacer |
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| Anmerkungen: |
Date Completed 13.03.2012 Date Revised 01.12.2018 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.ijfoodmicro.2011.08.007 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM211333654 |
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| 100 | 1 | |a Valera, Maria José |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Diversity of acetic acid bacteria present in healthy grapes from the Canary Islands |
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| 500 | |a Date Revised 01.12.2018 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2011 Elsevier B.V. All rights reserved. | ||
| 520 | |a The identification of acetic acid bacteria (AAB) from sound grapes from the Canary Islands is reported in the present study. No direct recovery of bacteria was possible in the most commonly used medium, so microvinifications were performed on grapes from Tenerife, La Palma and Lanzarote islands. Up to 396 AAB were isolated from those microvinifications and identified by 16S rRNA gene sequencing and phylogenetic analysis. With this method, Acetobacter pasteurianus, Acetobacter tropicalis, Gluconobacter japonicus and Gluconacetobacter saccharivorans were identified. However, no discrimination between the closely related species Acetobacter malorum and Acetobacter cerevisiae was possible. As previously described, 16S-23S rRNA gene internal transcribed spacer (ITS) region phylogenetic analysis was required to classify isolates as one of those species. These two species were the most frequently occurring, accounting for more than 60% of the isolates. For typing the AAB isolates, both the Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR and (GTG)5-PCR techniques gave similar resolution. A total of 60 profiles were identified. Thirteen of these profiles were found in more than one vineyard, and only one profile was found on two different islands (Tenerife and La Palma) | ||
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| 700 | 1 | |a Laich, Federico |e verfasserin |4 aut | |
| 700 | 1 | |a González, Sara S |e verfasserin |4 aut | |
| 700 | 1 | |a Torija, Maria Jesús |e verfasserin |4 aut | |
| 700 | 1 | |a Mateo, Estibaliz |e verfasserin |4 aut | |
| 700 | 1 | |a Mas, Albert |e verfasserin |4 aut | |
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