Insulin-like growth factor receptor I signaling in a breast cancer cell line
INTRODUCTION: In vitro studies strongly suggest that proliferation, migration and cell survival of breast cancer cell lines are significantly affected by activation of the IGF type 1 receptor (IGF-IR).
OBJECTIVE: The phosphorylation by IGF-I of IGF-IR and the intracellular signaling molecules Akt (PI-3K pathway) and Erk1/2 (MAPK pathway) was characterized in a human breast cancer cell lines.
MATERIALS AND METHODS: The study compared a standard breast adenocarcinoma line (MCF-7) cell line with a line (CSC 1595) derived from an infiltrating ductal breast cancer in a Colombian patient. The CSC 1595 and MCF-7 cell lines were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin and grown at 37 °C in 5% CO₂ atmosphere and 95% humidity. Cell extracts were prepared, followed by immunoprecipitation and immunoblotting with specific anti-pIGF-IR, anti-pERK1/2 and anti-pAkt antibodies.
RESULTS: After 5 minute stimulation with IGF-I, 70% of the IGF-IR was phosphorylated in the cell line CSC 1595 and 25% in MCF-7. In addition, Akt (oncogene protein v-akt) and ERK1/2 (extracellular signal-regulated MAP kinases) were phosphorylated. Basal and stimulated levels of phosphorylated ERK1/2 were substantially higher compared to those in the MCF-7 cell line.
CONCLUSIONS: The IGF-IR and MAPK kinase pathway involving proteins ERK1/2 showed more significant phosphorylation in the 1,595 cells compared to the observed in the MCF-7 cell line. Since the IGF-IR is the major activator of this pathway it may play an important role in ductal infiltrating breast cancer tumor growth and metastases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:30 |
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| Enthalten in: |
Biomedica : revista del Instituto Nacional de Salud - 30(2010), 4 vom: 21. Okt., Seite 551-8 |
| Sprache: |
Spanisch |
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| Weiterer Titel: |
Señalización asociada al receptor del factor de crecimiento similar a la insulina de tipo I en una línea celular colombiana de carcinoma mamario |
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| Beteiligte Personen: |
Mejía, Wilson [VerfasserIn] |
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| Anmerkungen: |
Date Completed 24.10.2011 Date Revised 29.06.2011 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM209551577 |
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| 100 | 1 | |a Mejía, Wilson |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Insulin-like growth factor receptor I signaling in a breast cancer cell line |
| 246 | 3 | 3 | |a Señalización asociada al receptor del factor de crecimiento similar a la insulina de tipo I en una línea celular colombiana de carcinoma mamario |
| 264 | 1 | |c 2010 | |
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| 500 | |a Date Revised 29.06.2011 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a INTRODUCTION: In vitro studies strongly suggest that proliferation, migration and cell survival of breast cancer cell lines are significantly affected by activation of the IGF type 1 receptor (IGF-IR) | ||
| 520 | |a OBJECTIVE: The phosphorylation by IGF-I of IGF-IR and the intracellular signaling molecules Akt (PI-3K pathway) and Erk1/2 (MAPK pathway) was characterized in a human breast cancer cell lines | ||
| 520 | |a MATERIALS AND METHODS: The study compared a standard breast adenocarcinoma line (MCF-7) cell line with a line (CSC 1595) derived from an infiltrating ductal breast cancer in a Colombian patient. The CSC 1595 and MCF-7 cell lines were cultured in DMEM supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, and 100 µg/ml streptomycin and grown at 37 °C in 5% CO₂ atmosphere and 95% humidity. Cell extracts were prepared, followed by immunoprecipitation and immunoblotting with specific anti-pIGF-IR, anti-pERK1/2 and anti-pAkt antibodies | ||
| 520 | |a RESULTS: After 5 minute stimulation with IGF-I, 70% of the IGF-IR was phosphorylated in the cell line CSC 1595 and 25% in MCF-7. In addition, Akt (oncogene protein v-akt) and ERK1/2 (extracellular signal-regulated MAP kinases) were phosphorylated. Basal and stimulated levels of phosphorylated ERK1/2 were substantially higher compared to those in the MCF-7 cell line | ||
| 520 | |a CONCLUSIONS: The IGF-IR and MAPK kinase pathway involving proteins ERK1/2 showed more significant phosphorylation in the 1,595 cells compared to the observed in the MCF-7 cell line. Since the IGF-IR is the major activator of this pathway it may play an important role in ductal infiltrating breast cancer tumor growth and metastases | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Phosphatidylinositol 3-Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.1.- |2 NLM | |
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| 650 | 7 | |a Extracellular Signal-Regulated MAP Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.11.24 |2 NLM | |
| 700 | 1 | |a Castro, Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Umaña, Adriana |e verfasserin |4 aut | |
| 700 | 1 | |a de Castro, Clemencia |e verfasserin |4 aut | |
| 700 | 1 | |a Riveros, Tulia |e verfasserin |4 aut | |
| 700 | 1 | |a Sánchez-Gómez, Myriam |e verfasserin |4 aut | |
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