A genetic algorithm for the optimization of the thermoinduction protocol for high-level production of recombinant human-like collagen from Escherichia coli
Copyright © 2011 International Union of Biochemistry and Molecular Biology, Inc..
Production of recombinant human-like collagen (RHLC) by thermoinduction of recombinant Escherichia coli BL 21 during high cell density cultivation was investigated in a 30 L bioreactor. The effects of induction temperature (T), pH, and carbon-to-nitrogen molar ratio of the nutrient medium (C/N) were examined. The optimal thermoinduction protocol for RHLC production was determined by using a model coupling genetic algorithm and artificial neural networks. The optimal operating conditions were as follows: maintenance of induction temperature at 42°C for 3 H and then at 39.4°C until the end, induction pH at 7.03, and C/N at 4.8 (mol/mol). The theoretical maximum concentration of RHLC was 12.5 g/L, whereas the experimental value was 12.1 g/L under the optimal induction conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:58 |
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| Enthalten in: |
Biotechnology and applied biochemistry - 58(2011), 3 vom: 01. Mai, Seite 175-84 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chi, Lei [VerfasserIn] |
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| Links: |
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| Themen: |
9007-34-5 |
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| Anmerkungen: |
Date Completed 06.02.2012 Date Revised 17.06.2011 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/bab.25 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM209223200 |
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| 245 | 1 | 2 | |a A genetic algorithm for the optimization of the thermoinduction protocol for high-level production of recombinant human-like collagen from Escherichia coli |
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| 500 | |a Date Revised 17.06.2011 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2011 International Union of Biochemistry and Molecular Biology, Inc. | ||
| 520 | |a Production of recombinant human-like collagen (RHLC) by thermoinduction of recombinant Escherichia coli BL 21 during high cell density cultivation was investigated in a 30 L bioreactor. The effects of induction temperature (T), pH, and carbon-to-nitrogen molar ratio of the nutrient medium (C/N) were examined. The optimal thermoinduction protocol for RHLC production was determined by using a model coupling genetic algorithm and artificial neural networks. The optimal operating conditions were as follows: maintenance of induction temperature at 42°C for 3 H and then at 39.4°C until the end, induction pH at 7.03, and C/N at 4.8 (mol/mol). The theoretical maximum concentration of RHLC was 12.5 g/L, whereas the experimental value was 12.1 g/L under the optimal induction conditions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
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| 650 | 7 | |a 9007-34-5 |2 NLM | |
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| 700 | 1 | |a Ma, Xiao-Xuan |e verfasserin |4 aut | |
| 700 | 1 | |a Mi, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yan-E |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Chen-Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Xiao-Li |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Wen-Jiao |e verfasserin |4 aut | |
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