Genetic bases of the temperature-sensitive phenotype of a master donor virus used in live attenuated influenza vaccines : A/Leningrad/134/17/57 (H2N2)
Published by Elsevier Inc..
Trivalent live attenuated influenza vaccines whose type A components are based on cold-adapted A/Leningrad/134/17/57 (H2N2) (caLen17) master donor virus (MDV) have been successfully used in Russia for decades to control influenza. The vaccine virus comprises hemagglutinin and neuraminidase genes from the circulating viruses and the remaining six genes from the MDV. The latter confer temperature-sensitive (ts) and attenuated (att) phenotypes. The ts phenotype of the vaccine virus is a critical biological determinant of attenuation of virulence. We developed a plasmid-based reverse genetics system for MDV caLen17 to study the genetic basis of its ts phenotype. Mutations in the polymerase proteins PB1 and PB2 played a crucial role in the ts phenotype of MDV caLen17. In addition, we show that caLen17-specific ts mutations could impart the ts phenotype to the divergent PR8 virus, suggesting the feasibility of transferring the ts phenotype to new viruses of interest for vaccine development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2011 |
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| Erschienen: |
2011 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:412 |
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| Enthalten in: |
Virology - 412(2011), 2 vom: 10. Apr., Seite 297-305 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Isakova-Sivak, Irina [VerfasserIn] |
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| Links: |
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| Themen: |
EC 2.7.7.48 |
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| Anmerkungen: |
Date Completed 18.05.2011 Date Revised 11.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.virol.2011.01.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM205809286 |
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| 100 | 1 | |a Isakova-Sivak, Irina |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genetic bases of the temperature-sensitive phenotype of a master donor virus used in live attenuated influenza vaccines |b A/Leningrad/134/17/57 (H2N2) |
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| 500 | |a Date Revised 11.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Published by Elsevier Inc. | ||
| 520 | |a Trivalent live attenuated influenza vaccines whose type A components are based on cold-adapted A/Leningrad/134/17/57 (H2N2) (caLen17) master donor virus (MDV) have been successfully used in Russia for decades to control influenza. The vaccine virus comprises hemagglutinin and neuraminidase genes from the circulating viruses and the remaining six genes from the MDV. The latter confer temperature-sensitive (ts) and attenuated (att) phenotypes. The ts phenotype of the vaccine virus is a critical biological determinant of attenuation of virulence. We developed a plasmid-based reverse genetics system for MDV caLen17 to study the genetic basis of its ts phenotype. Mutations in the polymerase proteins PB1 and PB2 played a crucial role in the ts phenotype of MDV caLen17. In addition, we show that caLen17-specific ts mutations could impart the ts phenotype to the divergent PR8 virus, suggesting the feasibility of transferring the ts phenotype to new viruses of interest for vaccine development | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a Influenza Vaccines |2 NLM | |
| 650 | 7 | |a PB2 protein, Influenzavirus A |2 NLM | |
| 650 | 7 | |a Vaccines, Attenuated |2 NLM | |
| 650 | 7 | |a Viral Proteins |2 NLM | |
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| 700 | 1 | |a Chen, Li-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Matsuoka, Yumiko |e verfasserin |4 aut | |
| 700 | 1 | |a Voeten, J Theo M |e verfasserin |4 aut | |
| 700 | 1 | |a Kiseleva, Irina |e verfasserin |4 aut | |
| 700 | 1 | |a Heldens, Jacco G M |e verfasserin |4 aut | |
| 700 | 1 | |a den Bosch, Han van |e verfasserin |4 aut | |
| 700 | 1 | |a Klimov, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Rudenko, Larisa |e verfasserin |4 aut | |
| 700 | 1 | |a Cox, Nancy J |e verfasserin |4 aut | |
| 700 | 1 | |a Donis, Ruben O |e verfasserin |4 aut | |
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