Polymerase chain reaction-based identity assay for pathogenic turkey Eimeria
Diagnosis of turkey Eimeria infection by conventional parasitologic methods is challenging and, until now, no molecular tools existed that clearly distinguished the four widely recognized pathogenic species: Eimeria adenoeides, E. meleagrimitis, E. gallopavonis, and E. dispersa. In this study, the internal transcribed spacer region one (ITS-1) was amplified and sequenced from 23 conventionally characterized reference samples. Phylogenic analysis segregated samples into five distinct cluster groups. The ITS-1 region(s) within each cluster were of a particular length and shared from 96% to 100% identity, while amplified ITS-1 region(s) between clusters differed in length and shared only 10.6% to 49.7% sequence identity. In addition, we developed PCR primer sets as diagnostic tools capable of specifically identifying members of each of the five clusters.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Avian diseases - 54(2010), 4 vom: 20. Dez., Seite 1152-6 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cook, Stephanie M [VerfasserIn] |
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| Themen: |
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| Anmerkungen: |
Date Completed 03.03.2011 Date Revised 14.02.2011 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM205794289 |
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| 245 | 1 | 0 | |a Polymerase chain reaction-based identity assay for pathogenic turkey Eimeria |
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| 500 | |a Date Completed 03.03.2011 | ||
| 500 | |a Date Revised 14.02.2011 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Diagnosis of turkey Eimeria infection by conventional parasitologic methods is challenging and, until now, no molecular tools existed that clearly distinguished the four widely recognized pathogenic species: Eimeria adenoeides, E. meleagrimitis, E. gallopavonis, and E. dispersa. In this study, the internal transcribed spacer region one (ITS-1) was amplified and sequenced from 23 conventionally characterized reference samples. Phylogenic analysis segregated samples into five distinct cluster groups. The ITS-1 region(s) within each cluster were of a particular length and shared from 96% to 100% identity, while amplified ITS-1 region(s) between clusters differed in length and shared only 10.6% to 49.7% sequence identity. In addition, we developed PCR primer sets as diagnostic tools capable of specifically identifying members of each of the five clusters | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Higuchi, Deborrah S |e verfasserin |4 aut | |
| 700 | 1 | |a McGowan, Alexandria L |e verfasserin |4 aut | |
| 700 | 1 | |a Schrader, Joan S |e verfasserin |4 aut | |
| 700 | 1 | |a Withanage, G S K |e verfasserin |4 aut | |
| 700 | 1 | |a Francis, Michael J |e verfasserin |4 aut | |
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