Genotoxicity of organic bentonite particles in vitro
OBJECTIVE: To study the genotoxicity induced by organic bentonite particles in vitro.
METHODS: Human B lymphoblast cells (HMy2.CIR) were exposed to organic bentonite particles at the doses of 0, 1.88, 3.75, 7.50 and 15.00 µg/ml for 24, 48 and 72 h, calcium sulfate (30 µg/ml) and SiO2 (30 and 240 µg/ml) served as negative and positive controls, respectively. The genotoxicity of organic bentonite particles and soluble fraction was detected using comet assay and Cytokinesis-block micronucleus (CBMN) assay.
RESULTS: The results of comet assay indicated that % tail DNA increased with the exposure doses and time in organic bentonite group, % tail DNA at the dose of 15.00 µg/ml for 24 h, 48 h and 72 h in organic bentonite group were 3.20 ± 0.19, 4.63 ± 0.88 and 9.49 ± 1.31 respectively which were significantly higher than those in calcium sulfate group (1.40 ± 0.11, 1.37 ± 0.22 and 0.90 ± 0.16) and those in 30 µg/ml SiO2 group (1.83 ± 0.21, 1.41 ± 0.27 and 2.48 ± 0.25) (P < 0.01). The results of CBMN assay showed that micronucleus frequencies (MNF) in organic bentonite group (except for 1.88 µg/ml for 24 h) were significantly higher than those in 30 µg/ml calcium sulfate group (MNF for 24, 48 and 72 h were 1.33‰ ± 0.58‰, 1.33‰ ± 1.15‰ and 1.33‰ ± 0.58‰) and those in 30 µg/ml SiO2 group (2.00‰ ± 0.00‰, 1.68‰ ± 0.58‰ and 2.33‰ ± 0.58‰) (P < 0.01). The results of two assays demonstrated that the soluble fraction of organic bentonite did not induce the genotoxicity.
CONCLUSION: The organic bentonite dusts can induce the genotoxicity in vitro, which may be from the particle fraction.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:28 |
|---|---|
| Enthalten in: |
Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases - 28(2010), 12 vom: 20. Dez., Seite 914-7 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Li, Xiao-Xue [VerfasserIn] |
|---|
| Themen: |
1302-78-9 |
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| Anmerkungen: |
Date Completed 13.08.2012 Date Revised 18.01.2011 published: Print Citation Status MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM205120946 |
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| 041 | |a chi | ||
| 100 | 1 | |a Li, Xiao-Xue |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Genotoxicity of organic bentonite particles in vitro |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
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| 500 | |a Date Completed 13.08.2012 | ||
| 500 | |a Date Revised 18.01.2011 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To study the genotoxicity induced by organic bentonite particles in vitro | ||
| 520 | |a METHODS: Human B lymphoblast cells (HMy2.CIR) were exposed to organic bentonite particles at the doses of 0, 1.88, 3.75, 7.50 and 15.00 µg/ml for 24, 48 and 72 h, calcium sulfate (30 µg/ml) and SiO2 (30 and 240 µg/ml) served as negative and positive controls, respectively. The genotoxicity of organic bentonite particles and soluble fraction was detected using comet assay and Cytokinesis-block micronucleus (CBMN) assay | ||
| 520 | |a RESULTS: The results of comet assay indicated that % tail DNA increased with the exposure doses and time in organic bentonite group, % tail DNA at the dose of 15.00 µg/ml for 24 h, 48 h and 72 h in organic bentonite group were 3.20 ± 0.19, 4.63 ± 0.88 and 9.49 ± 1.31 respectively which were significantly higher than those in calcium sulfate group (1.40 ± 0.11, 1.37 ± 0.22 and 0.90 ± 0.16) and those in 30 µg/ml SiO2 group (1.83 ± 0.21, 1.41 ± 0.27 and 2.48 ± 0.25) (P < 0.01). The results of CBMN assay showed that micronucleus frequencies (MNF) in organic bentonite group (except for 1.88 µg/ml for 24 h) were significantly higher than those in 30 µg/ml calcium sulfate group (MNF for 24, 48 and 72 h were 1.33‰ ± 0.58‰, 1.33‰ ± 1.15‰ and 1.33‰ ± 0.58‰) and those in 30 µg/ml SiO2 group (2.00‰ ± 0.00‰, 1.68‰ ± 0.58‰ and 2.33‰ ± 0.58‰) (P < 0.01). The results of two assays demonstrated that the soluble fraction of organic bentonite did not induce the genotoxicity | ||
| 520 | |a CONCLUSION: The organic bentonite dusts can induce the genotoxicity in vitro, which may be from the particle fraction | ||
| 650 | 4 | |a English Abstract | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
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| 700 | 1 | |a Zhang, Mei-Bian |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Ye-Zhen |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Song-Xue |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Xing, Ming-Luan |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Hua |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Shi-Jie |e verfasserin |4 aut | |
| 700 | 1 | |a He, Ji-Liang |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases |d 1993 |g 28(2010), 12 vom: 20. Dez., Seite 914-7 |w (DE-627)NLM083854649 |x 1001-9391 |7 nnns |
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