Screening of harmine tolerance/degrading bacteria from camel rumen
OBJECTIVE: Peganum harmala, a famous traditional Chinese drug, contains a variety of alkaloids and toxic for many animals. Camels mainly live in desert or semi-desert areas, with the robust gastrointestine system in digesting various feed including toxic plants without disease symptoms.
METHODS: Camel rumen content was used as the inoculant to inoculate medium M98-5 which contains 100 mg x L(-1) harmin and cultivated for 5 days. Upto 5 subculturings, strains that could degrading or tolerant harmine were isolated. Their conversion activity was determined by thin-layer chromatography. The taxonomic position of the strains were identified based on 16S rRNA sequences analysis.
RESULTS: 15 out of the 29 isolates have harmine degrading activity. Most of the isolates are identified as the members of the Genera Lactobacillus (16 strains, 55%), Shigella (7 strains, 24%) and Bacillus (4 strains, 13.8%). Only one strain belong to genus Enterococcus and one belong to genus Megasphaera.
CONCLUSION: The results indicated that the harmine tolerance/degrading communities of camel rumen are limited and only Lactobacillus have harmine-degrading activity.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
|---|---|
| Enthalten in: |
Wei sheng wu xue bao = Acta microbiologica Sinica - 50(2010), 8 vom: 27. Aug., Seite 1001-7 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
An, Dengdi [VerfasserIn] |
|---|
| Themen: |
4FHH5G48T7 |
|---|
| Anmerkungen: |
Date Completed 26.10.2010 Date Revised 25.11.2016 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM202237273 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM202237273 | ||
| 003 | DE-627 | ||
| 005 | 20231223223925.0 | ||
| 007 | tu | ||
| 008 | 231223s2010 xx ||||| 00| ||chi c | ||
| 028 | 5 | 2 | |a pubmed24n0674.xml |
| 035 | |a (DE-627)NLM202237273 | ||
| 035 | |a (NLM)20931866 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 100 | 1 | |a An, Dengdi |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Screening of harmine tolerance/degrading bacteria from camel rumen |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 26.10.2010 | ||
| 500 | |a Date Revised 25.11.2016 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: Peganum harmala, a famous traditional Chinese drug, contains a variety of alkaloids and toxic for many animals. Camels mainly live in desert or semi-desert areas, with the robust gastrointestine system in digesting various feed including toxic plants without disease symptoms | ||
| 520 | |a METHODS: Camel rumen content was used as the inoculant to inoculate medium M98-5 which contains 100 mg x L(-1) harmin and cultivated for 5 days. Upto 5 subculturings, strains that could degrading or tolerant harmine were isolated. Their conversion activity was determined by thin-layer chromatography. The taxonomic position of the strains were identified based on 16S rRNA sequences analysis | ||
| 520 | |a RESULTS: 15 out of the 29 isolates have harmine degrading activity. Most of the isolates are identified as the members of the Genera Lactobacillus (16 strains, 55%), Shigella (7 strains, 24%) and Bacillus (4 strains, 13.8%). Only one strain belong to genus Enterococcus and one belong to genus Megasphaera | ||
| 520 | |a CONCLUSION: The results indicated that the harmine tolerance/degrading communities of camel rumen are limited and only Lactobacillus have harmine-degrading activity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Harmine |2 NLM | |
| 650 | 7 | |a 4FHH5G48T7 |2 NLM | |
| 700 | 1 | |a Zhu, Yanlei |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Yongxia |e verfasserin |4 aut | |
| 700 | 1 | |a Zeng, Xianchun |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Wei sheng wu xue bao = Acta microbiologica Sinica |d 1985 |g 50(2010), 8 vom: 27. Aug., Seite 1001-7 |w (DE-627)NLM012631655 |x 0001-6209 |7 nnns |
| 773 | 1 | 8 | |g volume:50 |g year:2010 |g number:8 |g day:27 |g month:08 |g pages:1001-7 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 50 |j 2010 |e 8 |b 27 |c 08 |h 1001-7 | ||