Evaluation of nitrate reductase assay for rapid detection of resistance to second-line drugs in Mycobacterium tuberculosis in a tertiary care hospital
Copyright © 2010 Elsevier Inc. All rights reserved..
The present study evaluated nitrate reductase assay (NRA) for rapid detection of resistance to 5 second-line drugs: kanamycin, ethionamide, ofloxacin, cycloserine, and para-aminosalicylic acid. Eighty-six multidrug-resistant clinical isolates of Mycobacterium tuberculosis were used to compare NRA with proportion method for rapid detection of resistance to second-line drugs. The sensitivity of the NRA for different drugs ranged from 86.4% to 100%, whereas the specificity ranged from 98.4% to 100%. Excellent agreement was found between the 2 tests with respect to their κ values. For 63% of the isolates, the results were available in 14 days (turnaround time). The NRA is a rapid, accurate, simple, and inexpensive method for detection resistance to second-line drugs. This method may become an appropriate alternative method, especially for the resource poor settings.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:68 |
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| Enthalten in: |
Diagnostic microbiology and infectious disease - 68(2010), 3 vom: 15. Nov., Seite 236-40 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Visalakshi, Potharaju [VerfasserIn] |
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| Links: |
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| Themen: |
Antitubercular Agents |
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| Anmerkungen: |
Date Completed 24.01.2011 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.diagmicrobio.2010.06.017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM201665239 |
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| 520 | |a Copyright © 2010 Elsevier Inc. All rights reserved. | ||
| 520 | |a The present study evaluated nitrate reductase assay (NRA) for rapid detection of resistance to 5 second-line drugs: kanamycin, ethionamide, ofloxacin, cycloserine, and para-aminosalicylic acid. Eighty-six multidrug-resistant clinical isolates of Mycobacterium tuberculosis were used to compare NRA with proportion method for rapid detection of resistance to second-line drugs. The sensitivity of the NRA for different drugs ranged from 86.4% to 100%, whereas the specificity ranged from 98.4% to 100%. Excellent agreement was found between the 2 tests with respect to their κ values. For 63% of the isolates, the results were available in 14 days (turnaround time). The NRA is a rapid, accurate, simple, and inexpensive method for detection resistance to second-line drugs. This method may become an appropriate alternative method, especially for the resource poor settings | ||
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