Functionalized ZnO nanorod-based selective magnesium ion sensor for intracellular measurements
Copyright © 2010 Elsevier B.V. All rights reserved..
ZnO nanorods were grown on a silver-coated tip of a borosilicate glass capillary (0.7 μm in tip diameter) and used as selective potentiometric sensor of intracellular free Mg(2+). To functionalize the ZnO nanorods for selectivity of Mg(2+), a polymeric membrane with Mg(2+)-selective ionophores were coated on the surface of the ZnO nanorods. These functionalized ZnO nanorods exhibited a Mg(2+)-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode within the concentration range from 500 nM to 100 mM. Two types of cells, human adipocytes and frog oocytes, were used for the intracellular Mg(2+) measurements. The intracellular concentration of free Mg(2+) in human adipocytes and frog oocytes were 0.4-0.5 and 0.8-0.9 mM, respectively. Such type of nanoelectrode device paves the way to enable analytical measurements in single living cells and to sense other bio-chemical species at the intracellular level.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
Biosensors & bioelectronics - 26(2010), 3 vom: 15. Nov., Seite 1118-23 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Asif, Muhammad H [VerfasserIn] |
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| Links: |
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| Themen: |
Cations, Divalent |
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| Anmerkungen: |
Date Completed 03.02.2011 Date Revised 10.12.2019 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bios.2010.08.017 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a published: Print-Electronic | ||
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| 520 | |a Copyright © 2010 Elsevier B.V. All rights reserved. | ||
| 520 | |a ZnO nanorods were grown on a silver-coated tip of a borosilicate glass capillary (0.7 μm in tip diameter) and used as selective potentiometric sensor of intracellular free Mg(2+). To functionalize the ZnO nanorods for selectivity of Mg(2+), a polymeric membrane with Mg(2+)-selective ionophores were coated on the surface of the ZnO nanorods. These functionalized ZnO nanorods exhibited a Mg(2+)-dependent electrochemical potential difference versus an Ag/AgCl reference microelectrode within the concentration range from 500 nM to 100 mM. Two types of cells, human adipocytes and frog oocytes, were used for the intracellular Mg(2+) measurements. The intracellular concentration of free Mg(2+) in human adipocytes and frog oocytes were 0.4-0.5 and 0.8-0.9 mM, respectively. Such type of nanoelectrode device paves the way to enable analytical measurements in single living cells and to sense other bio-chemical species at the intracellular level | ||
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| 700 | 1 | |a Nur, Omer |e verfasserin |4 aut | |
| 700 | 1 | |a Willander, Magnus |e verfasserin |4 aut | |
| 700 | 1 | |a Englund, Ulrika H |e verfasserin |4 aut | |
| 700 | 1 | |a Elinder, Fredrik |e verfasserin |4 aut | |
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