Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion
Copyright © 2010 Elsevier Ltd. All rights reserved..
OBJECTIVE: To investigate the effects of experimentally created disordered occlusion on the mandibular condylar cartilage in terms of histological morphology and expression of MMP-3, MMP-9, TIMP-1 and aggrecan.
MATERIALS AND METHODS: Eighty 8-week-old Sprague-Dawley rats were randomly divided into two experimental (Exp) and two control (Con) groups, with equal sex and number distribution as subgroups. In the Exp group, the disordered occlusion was created by orthodontically moving the first and third molars 0.8mm away. Hematoxylin-eosin and immunohistochemical staining were performed on the mandibular condyles at the end of the 8th or 12th week. Gene expression was analysed by real-time PCR.
RESULTS: Osteoarthritis-like lesions, typically seen as a cell-free area, were detected in the Exp group, predominantly in females. In the cell-free area, the immunopositive expression of MMP-3, MMP-9, TIMP-1 and aggrecan were absent. Hyper-proliferation changes, typically seen as conjunctive invaginations of chondrocytes, were also observed where immunopositive expression of the tested materials was strong. There were sex and time point related differences in gene expression. In the 8-week subgroup, the expression of MMP-3 decreased, while aggrecan increased in males; however, both MMP-9 and TIMP increased in the female group (P<0.05). In the 12-week subgroup, the expression of MMP-3 increased, while TIMP, MMP-9 (male only) and aggrecan (female only) decreased (P<0.05).
CONCLUSIONS: The present results indicate that the experimentally created disordered occlusion led to osteoarthritis-like lesions accompanied by changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in mandibular condyle cartilage with gender differences.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
|---|---|
| Enthalten in: |
Archives of oral biology - 55(2010), 11 vom: 21. Nov., Seite 887-95 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Guo-Wei [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Aggrecans |
|---|
| Anmerkungen: |
Date Completed 04.02.2011 Date Revised 11.10.2010 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.archoralbio.2010.07.013 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM200489364 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM200489364 | ||
| 003 | DE-627 | ||
| 005 | 20231223220653.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.archoralbio.2010.07.013 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0668.xml |
| 035 | |a (DE-627)NLM200489364 | ||
| 035 | |a (NLM)20728870 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Guo-Wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in the condylar cartilage of rats induced by experimentally created disordered occlusion |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.02.2011 | ||
| 500 | |a Date Revised 11.10.2010 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2010 Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVE: To investigate the effects of experimentally created disordered occlusion on the mandibular condylar cartilage in terms of histological morphology and expression of MMP-3, MMP-9, TIMP-1 and aggrecan | ||
| 520 | |a MATERIALS AND METHODS: Eighty 8-week-old Sprague-Dawley rats were randomly divided into two experimental (Exp) and two control (Con) groups, with equal sex and number distribution as subgroups. In the Exp group, the disordered occlusion was created by orthodontically moving the first and third molars 0.8mm away. Hematoxylin-eosin and immunohistochemical staining were performed on the mandibular condyles at the end of the 8th or 12th week. Gene expression was analysed by real-time PCR | ||
| 520 | |a RESULTS: Osteoarthritis-like lesions, typically seen as a cell-free area, were detected in the Exp group, predominantly in females. In the cell-free area, the immunopositive expression of MMP-3, MMP-9, TIMP-1 and aggrecan were absent. Hyper-proliferation changes, typically seen as conjunctive invaginations of chondrocytes, were also observed where immunopositive expression of the tested materials was strong. There were sex and time point related differences in gene expression. In the 8-week subgroup, the expression of MMP-3 decreased, while aggrecan increased in males; however, both MMP-9 and TIMP increased in the female group (P<0.05). In the 12-week subgroup, the expression of MMP-3 increased, while TIMP, MMP-9 (male only) and aggrecan (female only) decreased (P<0.05) | ||
| 520 | |a CONCLUSIONS: The present results indicate that the experimentally created disordered occlusion led to osteoarthritis-like lesions accompanied by changes in the expression of MMP-3, MMP-9, TIMP-1 and aggrecan in mandibular condyle cartilage with gender differences | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Aggrecans |2 NLM | |
| 650 | 7 | |a Tissue Inhibitor of Metalloproteinase-1 |2 NLM | |
| 650 | 7 | |a Matrix Metalloproteinase 3 |2 NLM | |
| 650 | 7 | |a EC 3.4.24.17 |2 NLM | |
| 650 | 7 | |a Matrix Metalloproteinase 9 |2 NLM | |
| 650 | 7 | |a EC 3.4.24.35 |2 NLM | |
| 700 | 1 | |a Wang, Mei-Qing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiao-Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Shi-Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Xiao-Dong |e verfasserin |4 aut | |
| 700 | 1 | |a Jiao, Kai |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Archives of oral biology |d 1959 |g 55(2010), 11 vom: 21. Nov., Seite 887-95 |w (DE-627)NLM000027065 |x 1879-1506 |7 nnns |
| 773 | 1 | 8 | |g volume:55 |g year:2010 |g number:11 |g day:21 |g month:11 |g pages:887-95 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.archoralbio.2010.07.013 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 55 |j 2010 |e 11 |b 21 |c 11 |h 887-95 | ||