Are ankle chondrocytes from damaged fragments a suitable cell source for cartilage repair?
Copyright 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved..
OBJECTIVE: To characterize the post-expansion cartilage-forming capacity of chondrocytes harvested from detached fragments of osteochondral lesions (OCLs) of ankle joints (Damaged Ankle Cartilage Fragments, DACF), with normal ankle cartilage (NAC) as control.
DESIGN: DACF were obtained from six patients (mean age: 35 years) with symptomatic OCLs of the talus, while NAC were from 10 autopsies (mean age: 55 years). Isolated chondrocytes were expanded for two passages and then cultured in pellets for 14 days or onto HYAFF-11 meshes (FAB, Italy) for up to 28 days. Resulting tissues were assessed histologically, biochemically [glycosaminoglycan (GAG), DNA and type II collagen (CII)] and biomechanically.
RESULTS: As compared to NAC, DACF contained significantly lower amounts of DNA (3.0-fold), GAG (5.3-fold) and CII (1.5-fold) and higher amounts of type I collagen (6.2-fold). Following 14 days of culture in pellets, DACF-chondrocytes generated tissues less intensely stained for Safranin-O and CII, with significantly lower GAG contents (2.8-fold). After 28 days of culture onto HYAFF((R))-11, tissues generated by DACF-chondrocytes were less intensely stained for Safranin-O and CII, contained significantly lower amounts of GAG (1.9-fold) and CII (1.4-fold) and had lower equilibrium (1.7-fold) and dynamic pulsatile modulus (3.3-fold) than NAC-chondrocytes.
CONCLUSION: We demonstrated that DACF-chondrocytes have inferior cartilage-forming capacity as compared to NAC-chondrocytes, possibly resulting from environmental changes associated with trauma/disease. The study opens some reservations on the use of DACF-derived cells for the repair of ankle cartilage defects, especially in the context of tissue engineering-based approaches.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2010 |
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| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Osteoarthritis and cartilage - 18(2010), 8 vom: 15. Aug., Seite 1067-76 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Candrian, C [VerfasserIn] |
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| Anmerkungen: |
Date Completed 14.03.2011 Date Revised 18.10.2016 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.joca.2010.04.010 |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Are ankle chondrocytes from damaged fragments a suitable cell source for cartilage repair? |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved. | ||
| 520 | |a OBJECTIVE: To characterize the post-expansion cartilage-forming capacity of chondrocytes harvested from detached fragments of osteochondral lesions (OCLs) of ankle joints (Damaged Ankle Cartilage Fragments, DACF), with normal ankle cartilage (NAC) as control | ||
| 520 | |a DESIGN: DACF were obtained from six patients (mean age: 35 years) with symptomatic OCLs of the talus, while NAC were from 10 autopsies (mean age: 55 years). Isolated chondrocytes were expanded for two passages and then cultured in pellets for 14 days or onto HYAFF-11 meshes (FAB, Italy) for up to 28 days. Resulting tissues were assessed histologically, biochemically [glycosaminoglycan (GAG), DNA and type II collagen (CII)] and biomechanically | ||
| 520 | |a RESULTS: As compared to NAC, DACF contained significantly lower amounts of DNA (3.0-fold), GAG (5.3-fold) and CII (1.5-fold) and higher amounts of type I collagen (6.2-fold). Following 14 days of culture in pellets, DACF-chondrocytes generated tissues less intensely stained for Safranin-O and CII, with significantly lower GAG contents (2.8-fold). After 28 days of culture onto HYAFF((R))-11, tissues generated by DACF-chondrocytes were less intensely stained for Safranin-O and CII, contained significantly lower amounts of GAG (1.9-fold) and CII (1.4-fold) and had lower equilibrium (1.7-fold) and dynamic pulsatile modulus (3.3-fold) than NAC-chondrocytes | ||
| 520 | |a CONCLUSION: We demonstrated that DACF-chondrocytes have inferior cartilage-forming capacity as compared to NAC-chondrocytes, possibly resulting from environmental changes associated with trauma/disease. The study opens some reservations on the use of DACF-derived cells for the repair of ankle cartilage defects, especially in the context of tissue engineering-based approaches | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Miot, S |e verfasserin |4 aut | |
| 700 | 1 | |a Wolf, F |e verfasserin |4 aut | |
| 700 | 1 | |a Bonacina, E |e verfasserin |4 aut | |
| 700 | 1 | |a Dickinson, S |e verfasserin |4 aut | |
| 700 | 1 | |a Wirz, D |e verfasserin |4 aut | |
| 700 | 1 | |a Jakob, M |e verfasserin |4 aut | |
| 700 | 1 | |a Valderrabano, V |e verfasserin |4 aut | |
| 700 | 1 | |a Barbero, A |e verfasserin |4 aut | |
| 700 | 1 | |a Martin, I |e verfasserin |4 aut | |
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