Quantitative measurement of mature collagen cross-links in human carotid artery plaques
Copyright 2010 Elsevier Ireland Ltd. All rights reserved..
While there is an appreciable understanding of the importance of collagen breakdown in contributing to atherosclerotic plaque vulnerability and rupture, little is known about changes in collagen maturation in the atherosclerotic plaque. This is achieved through the formation of the covalent intermolecular cross-links pyridinoline (Pyd) and deoxypyridinoline (Dpd). In this study we collected carotid endarterectomy specimens from patients and undertook (i) histological assessment of collagen and inflammatory cell distribution and (ii) biochemical analysis of total collagen and cross-link content. Greater collagen deposition, increased presence of CD68 positive cells and an increased Pyd:Dpd ratio (an indicator of lysyl hydroxylase (LH-1) activity) were found in plaque versus normal vascular tissue. These findings are the first measurements of Pyd and Dpd cross-links in normal and atherosclerotic vascular tissue. The observed differences in cross-links in the plaque may adversely affect tensile strength and may have relevance to the mechanisms underlying rupture of vulnerable plaques.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2010 |
|---|---|
| Erschienen: |
2010 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:211 |
|---|---|
| Enthalten in: |
Atherosclerosis - 211(2010), 2 vom: 16. Aug., Seite 471-4 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hector, Emma E [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 06.12.2010 Date Revised 02.08.2010 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.atherosclerosis.2010.03.028 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM197590004 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM197590004 | ||
| 003 | DE-627 | ||
| 005 | 20231223210908.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231223s2010 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.atherosclerosis.2010.03.028 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n0659.xml |
| 035 | |a (DE-627)NLM197590004 | ||
| 035 | |a (NLM)20417515 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hector, Emma E |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Quantitative measurement of mature collagen cross-links in human carotid artery plaques |
| 264 | 1 | |c 2010 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 06.12.2010 | ||
| 500 | |a Date Revised 02.08.2010 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright 2010 Elsevier Ireland Ltd. All rights reserved. | ||
| 520 | |a While there is an appreciable understanding of the importance of collagen breakdown in contributing to atherosclerotic plaque vulnerability and rupture, little is known about changes in collagen maturation in the atherosclerotic plaque. This is achieved through the formation of the covalent intermolecular cross-links pyridinoline (Pyd) and deoxypyridinoline (Dpd). In this study we collected carotid endarterectomy specimens from patients and undertook (i) histological assessment of collagen and inflammatory cell distribution and (ii) biochemical analysis of total collagen and cross-link content. Greater collagen deposition, increased presence of CD68 positive cells and an increased Pyd:Dpd ratio (an indicator of lysyl hydroxylase (LH-1) activity) were found in plaque versus normal vascular tissue. These findings are the first measurements of Pyd and Dpd cross-links in normal and atherosclerotic vascular tissue. The observed differences in cross-links in the plaque may adversely affect tensile strength and may have relevance to the mechanisms underlying rupture of vulnerable plaques | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Amino Acids |2 NLM | |
| 650 | 7 | |a Antigens, CD |2 NLM | |
| 650 | 7 | |a Antigens, Differentiation, Myelomonocytic |2 NLM | |
| 650 | 7 | |a CD68 antigen, human |2 NLM | |
| 650 | 7 | |a Cross-Linking Reagents |2 NLM | |
| 650 | 7 | |a pyridinoline |2 NLM | |
| 650 | 7 | |a 63800-01-1 |2 NLM | |
| 650 | 7 | |a deoxypyridinoline |2 NLM | |
| 650 | 7 | |a 90032-33-0 |2 NLM | |
| 650 | 7 | |a Collagen |2 NLM | |
| 650 | 7 | |a 9007-34-5 |2 NLM | |
| 650 | 7 | |a Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase |2 NLM | |
| 650 | 7 | |a EC 1.14.11.4 |2 NLM | |
| 700 | 1 | |a Robins, Simon P |e verfasserin |4 aut | |
| 700 | 1 | |a Mercer, Derry K |e verfasserin |4 aut | |
| 700 | 1 | |a Brittenden, Julie |e verfasserin |4 aut | |
| 700 | 1 | |a Wainwright, Cherry L |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Atherosclerosis |d 1970 |g 211(2010), 2 vom: 16. Aug., Seite 471-4 |w (DE-627)NLM000087394 |x 1879-1484 |7 nnns |
| 773 | 1 | 8 | |g volume:211 |g year:2010 |g number:2 |g day:16 |g month:08 |g pages:471-4 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.atherosclerosis.2010.03.028 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 211 |j 2010 |e 2 |b 16 |c 08 |h 471-4 | ||